Departamento de Producción Animal, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain.
Zoonoses Public Health. 2012 Sep;59(6):401-7. doi: 10.1111/j.1863-2378.2012.01467.x. Epub 2012 Feb 21.
The risk of zoonoses is a major obstacle to xenotransplantation. Porcine endogenous retrovirus (PERV) poses a potential risk of zoonotic infection, and its control is a prerequisite for the development of clinical xenotransplantation. The copy number of PERV varies among different breeds, and it has been suggested that the PERV integrations number is increased by inbreeding. The purpose of this study was (i) to examine the copy number of PERV in different Spanish pig breeds, Spanish wild boar and commercial cross-bred pigs from five different farms and genetic background (CCP1-CCP5) and (ii) to investigate the correlation between PERV copy number and the genetic background of the pigs in order to improve the selection of pigs for xenotransplantation. PERV copy number was determined by quantitative, real-time polymerase chain reactions. Thirty-four microsatellite markers were genotyped to describe the genetic diversity within populations (observed and expected heterozygosities, Ho and He, respectively) and the inbreeding coefficient (F). Pearson's correlation coefficient was used to determine the relationship between PERV copy number and Ho, He and F. The copy number of PERV among different pig breeds was estimated to range between three (CCP1) and 43 copies (Iberian Pig). Statistical differences were found among the studied populations concerning PERV copy number. No correlation was found between the PERV copy number and the heterozygosity (calculated at an individual level or at a population level) or the inbreeding coefficient of each population. Our data suggest that pigs inbreeding does not increase PERV copy number and support the idea that careful selection of pigs for organ donation with reduced PERV copy number will minimize the risk of retrovirus transmission to the human receptor.
人畜共患病的风险是异种移植的主要障碍。猪内源性逆转录病毒 (PERV) 存在潜在的人畜共患病感染风险，其控制是临床异种移植发展的前提。不同品种的 PERV 拷贝数存在差异，有人认为，近交增加了 PERV 的整合数。本研究的目的是：(i) 检测来自五个不同农场和遗传背景（CCP1-CCP5）的不同西班牙猪品种、西班牙野猪和商业杂交猪的 PERV 拷贝数；(ii) 研究 PERV 拷贝数与猪遗传背景的相关性，以便改进异种移植猪的选择。通过定量实时聚合酶链反应确定 PERV 拷贝数。对 34 个微卫星标记进行基因分型，以描述群体内的遗传多样性（观察和预期杂合度，分别为 Ho 和 He）和近交系数（F）。使用 Pearson 相关系数确定 PERV 拷贝数与 Ho、He 和 F 之间的关系。不同猪品种之间的 PERV 拷贝数估计在 3 个（CCP1）到 43 个拷贝（伊比利亚猪）之间。研究人群之间的 PERV 拷贝数存在统计学差异。在个体水平或群体水平上计算的杂合度或每个群体的近交系数与 PERV 拷贝数之间没有相关性。我们的数据表明，猪近交不会增加 PERV 拷贝数，并支持这样一种观点，即仔细选择 PERV 拷贝数降低的猪作为器官捐献者，将最大限度地降低将逆转录病毒传播给人类受体的风险。
