Gołos Barbara, Dąbrowska Magdalena, Wałajtys-Rode Elżbieta, Zieliński Zbigniew, Wińska Patrycja, Cieśla Joanna, Jagielska Elżbieta, Moczoń Tadeusz, Rode Wojciech
Nencki Institute of Experimental Biology, Polish Academy of Sciences, 02-093 Warszawa, Poland.
Mol Biochem Parasitol. 2012 May;183(1):63-9. doi: 10.1016/j.molbiopara.2012.02.002. Epub 2012 Feb 13.
Localization of thymidylate synthase protein in Trichinella spiralis and Caenorhabditis elegans development was followed with the use of confocal microscopy, revealing similar expression patterns in both nematode species. In T. spiralis premature muscle larvae and C. elegans dauer, L3 and L4 larvae, thymidylate synthase was detected in the nerve ring and gonad primordia, as well as T. spiralis stichosome and C. elegans pharyngeal glandular cells. In developmentally arrested T. spiralis muscle larvae, the enzyme was found localized to the gonad primordia and stichosome. High enzyme level was also observed in the embryos developing in uteri of T. spiralis female adult and C. elegans hermaphrodite forms. In the case of T. spiralis adult forms, thymidylate synthase was detected in stichosome, along esophagus wall, as well as in egg and sperm cells. While the enzyme protein present in the embryos remains in accord with its known association with proliferating systems, thymidylate synthase presence in the nerve ring, and reproductive and secretory (T. spiralis stichosomal and C. elegans pharyngeal glandular cells) systems, points to a state of cell cycle-arrest, also known to preserve the enzyme protein.
利用共聚焦显微镜观察了旋毛虫和秀丽隐杆线虫发育过程中胸苷酸合成酶蛋白的定位，结果显示这两种线虫的表达模式相似。在旋毛虫的早熟肌幼虫以及秀丽隐杆线虫的 dauer 幼虫、L3 和 L4 幼虫中，在神经环、性腺原基以及旋毛虫的杆状体和秀丽隐杆线虫的咽腺细胞中均检测到了胸苷酸合成酶。在发育停滞的旋毛虫肌幼虫中，该酶定位于性腺原基和杆状体。在旋毛虫雌成虫和秀丽隐杆线虫雌雄同体形态的子宫内发育的胚胎中也观察到了高酶水平。对于旋毛虫成虫形态，在杆状体、食管壁以及卵细胞和精子细胞中检测到了胸苷酸合成酶。虽然胚胎中存在的酶蛋白与其已知的与增殖系统的关联一致，但胸苷酸合成酶在神经环以及生殖和分泌（旋毛虫杆状体和秀丽隐杆线虫咽腺细胞）系统中的存在，表明细胞周期停滞状态，已知这种状态也能保留酶蛋白。
