State Key Laboratory of Cellular Stress Biology, School of Life Science, Xiamen University, Xiamen, Fujian 361005, People's Republic of China.
Gene. 2013 Jan 10;512(2):496-504. doi: 10.1016/j.gene.2012.10.010. Epub 2012 Oct 23.
A cDNA encoding mitochondrial ribosomal protein (MRP)-L28 was isolated and cloned from Trichinella spiralis, an economically important parasitic nematode. The predicted TsMRP-L28 protein consists of 276 amino acids. Phylogenetic analysis revealed that TsMRP-L28 was closely related to Caenorhabditis elegans mitochondrial ribosomal protein L28. The TsMRP-L28 transcript was expressed in newborn larvae, muscle larvae and male and female adult worms. Western blot showed that TsMRP-L28 was expressed in muscle larvae and adult worms. Immuno-localization revealed that TsMRP-L28 was ubiquitously distributed in newborn larvae, muscle larvae and adult worms, and that TsMRP-L28 was enriched in cells with higher protein synthesis activity, such as in newborn larvae and the cytoplasm of different developmental stages of embryos. These data suggest that TsMRP-L28 is required for the early development of T. spiralis.
从经济上重要的寄生线虫旋毛虫中分离并克隆了编码线粒体核糖体蛋白（MRP）-L28 的 cDNA。预测的 TsMRP-L28 蛋白由 276 个氨基酸组成。系统发育分析表明，TsMRP-L28 与秀丽隐杆线虫的线粒体核糖体蛋白 L28 密切相关。TsMRP-L28 转录本在新生幼虫、肌肉幼虫和雌雄成虫中表达。Western blot 显示 TsMRP-L28 在肌肉幼虫和成虫中表达。免疫定位显示 TsMRP-L28 在新生幼虫、肌肉幼虫和成虫中广泛分布，并且 TsMRP-L28 在蛋白质合成活性较高的细胞中富集，例如在新生幼虫和不同发育阶段胚胎的细胞质中。这些数据表明 TsMRP-L28 是旋毛虫早期发育所必需的。
