Dipartimento di Chimica, Università degli Studi di Firenze, 50121 Firenze, Italy.
Anal Bioanal Chem. 2012 Apr;402(10):3103-14. doi: 10.1007/s00216-012-5769-1. Epub 2012 Feb 16.
Recent progress in the development of electrochemical nanomaterial-aptamer-based biosensors is summarized. Aptamers are nucleic acid ligands that can be generated against amino acids, drugs, proteins, and other molecules. They are isolated from a large random library of synthetic nucleic acids by an iterative process of binding, separation, and amplification, called systematic evolution of ligands by exponential enrichment (SELEX). In this review, different methods of integrating aptamers with different nanomaterials and nanoparticles for electrochemical biosensing application are described.
本文总结了电化学纳米材料-适体生物传感器的最新进展。适体是可以针对氨基酸、药物、蛋白质和其他分子生成的核酸配体。它们是通过称为指数富集的配体系统进化(SELEX)的结合、分离和扩增的迭代过程从合成核酸的大型随机文库中分离出来的。在本文中,描述了将适体与用于电化学生物传感应用的不同纳米材料和纳米粒子进行集成的不同方法。