Dipartimento Produzioni Animali, Epidemiologia ed Ecologia, Università degli Studi di Torino, Grugliasco, Torino, Italy.
Exp Appl Acarol. 2012 Apr;56(4):391-401. doi: 10.1007/s10493-012-9528-y. Epub 2012 Feb 17.
Between May 2006 and January 2007, blood samples and ticks were randomly collected from 220 nomadic animals from Filtu and Dollo Odo districts, Libaan zone, in the Somali Region of Ethiopia. Overall, 81.5% cattle, 98.2% camels, 53.4% goats and 61.1% sheep were infested by ixodid ticks. Collected ticks (n = 1,036) were identified as Rhipicephalus pulchellus (40.1%), R. pravus (25.8%), Amblyomma gemma (9.4%), Hyalomma rufipes (13.3%), H. truncatum (2.8%), H. impeltatum (1.2%) and H. dromedarii (0.5%); immature stages (6.1%) belonged to the genera Rhipicephalus and Amblyomma. Tick infestation burden was evaluated by the Tick Abundance Score method on 57 animals from Dollo Odo in August 2006, and it was significantly higher in cattle and camels than in small ruminants (p < 0.001). Reverse Line Blot Hybridisation was applied to detect Theileria, Babesia, Ehrlichia and Anaplasma spp. Five out of 50 blood samples from Filtu, four from cattle and, surprisingly, one from a camel, were positive for Theileria mutans and two from cattle for T. velifera. Adult ticks (n = 104) from both districts were tested and A. gemma from cattle were positive to T. velifera (1) and Ehrlichia ruminantium (5 samples). Positive E. ruminantium samples were also tested by PCR targeting pCS20 and 16S rRNA genes and submitted to DNA sequencing. The phylogenetic reconstruction of pCS20 fragment showed the presence of the Somali region sequences in the East-South African group. Our results are the first available on ticks and selected tick-borne diseases from the Somali region of Ethiopia and could be used as preliminary information for planning sustainable control strategies for tick and tick-borne pathogens in the study area and in neighbouring areas with similar socio-ecological features.
2006 年 5 月至 2007 年 1 月期间，在埃塞俄比亚索马里地区的利班地区的菲尔图和多洛奥多区，随机采集了 220 头游牧动物的血液样本和蜱虫。总体而言，81.5%的牛、98.2%的骆驼、53.4%的山羊和 61.1%的绵羊都被硬蜱感染。采集的蜱虫（n=1036）被鉴定为 Rhipicephalus pulchellus（40.1%）、R. pravus（25.8%）、Amblyomma gemma（9.4%）、Hyalomma rufipes（13.3%）、H. truncatum（2.8%）、H. impeltatum（1.2%）和 H. dromedarii（0.5%）；未成熟阶段（6.1%）属于 Rhipicephalus 和 Amblyomma 属。2006 年 8 月，在多洛奥多的 57 头动物上评估了蜱虫丰度评分法的蜱虫感染负担，牛和骆驼的感染负担明显高于小反刍动物（p<0.001）。反向线杂交被用于检测泰勒虫、巴贝斯虫、埃立克体和无形体属。在菲尔图的 50 份血液样本中，有 5 份来自牛，令人惊讶的是，1 份来自骆驼，对曼氏泰勒虫呈阳性，还有 2 份来自牛，对泰勒虫 velifera 呈阳性。来自两个地区的成年蜱虫（n=104）进行了检测，来自牛的 A. gemma 对泰勒虫 velifera（1）和埃立克体 ruminantium（5 个样本）呈阳性。还对阳性埃立克体 ruminantium 样本进行了针对 pCS20 和 16S rRNA 基因的 PCR 检测，并提交 DNA 测序。pCS20 片段的系统发育重建显示，在东-南非组中存在索马里地区的序列。我们的结果是关于埃塞俄比亚索马里地区的蜱虫和选定的蜱传疾病的首批结果，可作为该研究区域和具有类似社会生态特征的邻近区域制定可持续的蜱虫和蜱传病原体控制策略的初步信息。
