Van Wouwe J P, Veldhuizen M, Van den Hamer C J, de Goeij J J
Department of Radiochemistry, Technical University, Delft, The Netherlands.
Pediatr Res. 1990 Oct;28(4):332-5. doi: 10.1203/00006450-199010000-00006.
To establish a parameter for zinc status that is independent of the occurrence of infection, we studied the effects of low dietary zinc and endotoxin in weaning rats 21 d after 65Zn intubation. We monitored aspects of zinc status (tissue zinc content, 65Zn distribution, and specific 65Zn activity in tissue) and 65Zn metabolism (absorption, excretion, and biologic half-life), as well as weight gain, feed conversion, and dietary zinc use. The low zinc diet induced classical deficiency with losses of bone zinc, resulting in lower content (7.4 versus 19.6 mumol) and higher spec act (17 versus 8 kBq/mumol). Other tissue-specific and plasma-specific activities were also higher (overall, 20 versus 8 kBq/mumol; plasma, 8 versus 4 kBq/mumol). Endotoxin caused lower total-plasma zinc (0.04 versus 0.05 mumol) but did not affect spec act (4 kBq/mumol); combined endotoxin and low-zinc diet caused low total-plasma zinc (0.01 mumol) and high spec act, as did the low-zinc diet alone (12 kBq/mumol). We conclude that plasma-spec act (or stable isotope enrichment) can serve as an index for nutritional zinc status during recurrent infection.
为建立一个独立于感染发生情况的锌状态参数，我们在给断奶大鼠插管注入65Zn 21天后，研究了低锌饮食和内毒素的影响。我们监测了锌状态的各个方面（组织锌含量、65Zn分布以及组织中的特定65Zn活性）和65Zn代谢（吸收、排泄以及生物半衰期），以及体重增加、饲料转化率和膳食锌利用情况。低锌饮食导致典型的锌缺乏，伴有骨锌流失，导致含量降低（7.4对19.6 μmol）和特定活性升高（17对8 kBq/μmol）。其他组织特异性和血浆特异性活性也更高（总体上，20对8 kBq/μmol；血浆，8对4 kBq/μmol）。内毒素导致血浆总锌含量降低（0.04对0.05 μmol），但不影响特定活性（4 kBq/μmol）；内毒素和低锌饮食联合导致血浆总锌含量低（0.01 μmol）和特定活性高，单独低锌饮食也是如此（12 kBq/μmol）。我们得出结论，血浆特定活性（或稳定同位素富集）可作为反复感染期间营养性锌状态的指标。
