Matherne G P, Headrick J P, Coleman S D, Berne R M
Department of Pediatrics, University of Virginia Health Sciences Center, Charlottesville 22908.
Pediatr Res. 1990 Oct;28(4):348-53. doi: 10.1203/00006450-199010000-00010.
Interstitial transudate and coronary venous concentrations of adenosine, inosine, and hypoxanthine were determined in isolated isovolumic immature and mature rabbit hearts during normoxia and hypoxia. During normoxia, interstitial transudate adenosine was lower in immature hearts compared with mature hearts. Interstitial transudate concentrations of adenosine, inosine, and hypoxanthine were 130 +/- 16 nM, 699 +/- 88 nM, and 392 +/- 80 nM, respectively, in immature rabbit hearts and 228 +/- 35 nM, 1154 +/- 126 nM, and 287 +/- 30 nM, respectively, in mature rabbit hearts. Interstitial transudate adenosine was significantly lower in the immature hearts. Coronary venous purine concentrations were 6- and 8-fold lower than their respective interstitial transudate concentrations during normoxia in both age groups. Hypoxia significantly increased interstitial transudate purines in both age groups. Interstitial transudate adenosine, inosine, and hypoxanthine increased to 1180 +/- 231 nM, 4049 +/- 500 nM, and 1099 +/- 98 nM, respectively, in immature hearts and to 1225 +/- 300 nM, 5220 +/- 1217 nM, and 876 +/- 147 nM, respectively, in mature hearts. The age-related difference in transudate adenosine levels present during normoxia was not detected during hypoxia. Venous purine levels increased during hypoxia and the gradient from interstitial transudate fluid to venous effluent was abolished for adenosine in both groups. In immature hearts, hypoxia led to higher venous effluent adenosine levels than in the mature hearts. Coronary resistance correlated with interstitial transudate adenosine in both groups, although immature hearts displayed lower resistances at all adenosine levels.(ABSTRACT TRUNCATED AT 250 WORDS)
在常氧和缺氧条件下，测定分离的等容未成熟和成熟兔心脏中腺苷、肌苷和次黄嘌呤的间质渗出液及冠状静脉浓度。常氧时，未成熟心脏间质渗出液中的腺苷低于成熟心脏。未成熟兔心脏间质渗出液中腺苷、肌苷和次黄嘌呤的浓度分别为130±16 nM、699±88 nM和392±80 nM，成熟兔心脏中分别为228±35 nM、1154±126 nM和287±30 nM。未成熟心脏中间质渗出液中的腺苷显著更低。两个年龄组在常氧时冠状静脉嘌呤浓度均比各自的间质渗出液浓度低6至8倍。缺氧显著增加了两个年龄组间质渗出液中的嘌呤。未成熟心脏中间质渗出液中的腺苷、肌苷和次黄嘌呤分别增至1180±231 nM、4049±500 nM和1099±98 nM，成熟心脏中分别增至1225±300 nM、5220±1217 nM和876±147 nM。缺氧时未检测到常氧时存在的渗出液腺苷水平的年龄相关差异。缺氧时静脉嘌呤水平升高，两组中腺苷从间质渗出液到静脉流出液的梯度均消失。在未成熟心脏中，缺氧导致静脉流出液中的腺苷水平高于成熟心脏。两组中冠状阻力均与间质渗出液中的腺苷相关，尽管在所有腺苷水平下未成熟心脏的阻力均较低。（摘要截断于250词）
