Fenton R A, Dobson J G
Circ Res. 1987 Feb;60(2):177-84. doi: 10.1161/01.res.60.2.177.
An improved assay was used to investigate the effects of hypoxia or ischemia on interstitial fluid and coronary venous effluent levels of adenosine in isolated perfused nonworking rat hearts. The adenosine in 5- to 10-microliter samples of left ventricular epicardial surface transudates and coronary effluents was reacted with chloroacetaldehyde, and the fluorescent derivative (1,N6-ethenoadenosine) was quantitated using high pressure liquid chromatography and fluorescence detection. Hearts responding to hypoxia could be separated into two groups. In one group of hearts, the control (normoxic) transudate and effluent adenosine concentrations were 94 +/- 24 and 41 +/- 6 pmol/ml, respectively. These values increased by 118 and 96%, respectively, with 5 minutes of hypoxia (30% O2), and returned to control levels 5 minutes after resumption of normoxia. In a second group of hearts, the normoxic control levels of adenosine in the transudates (42 +/- 7 pmol/ml) and coronary effluents (62 +/- 17 pmol/ml) were increased with hypoxia by 174 and 1,178%, respectively. However, the transudate levels continued to rise for 5 minutes after resumption of normoxic perfusion while effluent levels fell. In another series of hearts, global ischemia for 30 seconds elicited an elevation of transudate adenosine levels by 362 to 641% above control (58 +/- 15 pmol/ml) as determined 30 seconds after resumption of perfusion flow.(ABSTRACT TRUNCATED AT 250 WORDS)
采用一种改进的检测方法，研究缺氧或缺血对离体灌注的非工作大鼠心脏间质液和冠状静脉流出液中腺苷水平的影响。取5至10微升左心室心外膜表面渗出液和冠状流出液样本中的腺苷，与氯乙醛反应，使用高压液相色谱和荧光检测对荧光衍生物（1,N6-乙烯腺苷）进行定量。对缺氧有反应的心脏可分为两组。在一组心脏中，对照（常氧）渗出液和流出液中的腺苷浓度分别为94±24和41±6皮摩尔/毫升。在缺氧5分钟（30%氧气）时，这些值分别增加了118%和96%，恢复常氧5分钟后恢复到对照水平。在另一组心脏中，渗出液（42±7皮摩尔/毫升）和冠状流出液（62±17皮摩尔/毫升）中腺苷的常氧对照水平在缺氧时分别增加了174%和1178%。然而，恢复常氧灌注后，渗出液水平在5分钟内持续上升，而流出液水平下降。在另一系列心脏中，灌注流恢复30秒后测定，30秒的全心缺血使渗出液腺苷水平比对照（58±15皮摩尔/毫升）升高362%至641%。（摘要截断于250字）
