Core Facility-Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
J Agric Food Chem. 2012 Mar 14;60(10):2418-26. doi: 10.1021/jf204475e. Epub 2012 Mar 5.
Two-dimensional gel electrophoresis in combination with mass spectrometry has already been applied successfully to study beer proteome. Due to the abundance of protein Z in beer samples, prefractionation techniques might help to improve beer proteome coverage. Proteins from four lager beers of different origins were separated by two-dimensional electrophoresis (2-DE) followed by tandem mass spectrometric analysis. Initially 52 proteins mostly from Hordeum vulgare (22 proteins) and Saccharomyces species (25 proteins) were identified. Preparative isoelectric focusing by OFFGEL Fractionator was applied prior to 2-DE to improve its resolution power. As a result of this combined approach, a total of 70 beer proteins from Hordeum vulgare (30 proteins), from Saccharomyces species (31 proteins), and from other sources (9 proteins) were identified. Of these, 37 proteins have not been previously reported in beer samples.
二维凝胶电泳结合质谱已成功应用于研究啤酒蛋白质组。由于啤酒样品中蛋白质 Z 的丰度较高，预分级技术可能有助于提高啤酒蛋白质组的覆盖度。来自不同来源的四种拉格啤酒的蛋白质通过二维电泳（2-DE）分离，然后进行串联质谱分析。最初鉴定出 52 种蛋白质，主要来自大麦（22 种蛋白质）和酿酒酵母属（25 种蛋白质）。在 2-DE 之前应用 OFFGEL Fractionator 进行制备等电聚焦以提高其分辨率。通过这种联合方法，共鉴定出来自大麦（30 种蛋白质）、酿酒酵母属（31 种蛋白质）和其他来源（9 种蛋白质）的 70 种啤酒蛋白质。其中，37 种蛋白质以前未在啤酒样品中报道过。
