Beh K J, Buttery S H
Aust Vet J. 1978 Nov;54(11):541-4. doi: 10.1111/j.1751-0813.1978.tb00330.x.
Two in vitro immunological assays were developed for detection of the epsilon (epsilon) antigen of Cl. perfringens type D. It was found that the reverse phase passive haemagglutination assay (RPHA) was able to detect concentrations of epsilon-antigen as low as 6 x 10-7 mg/ml whereas the single radial immunodiffusion techniques (SRID) was capable of detecting concentrations of epsilon-antigen above 0.01 mg/ml. When applied to gut contents from freshly dead infected sheep the RPHA test was found to be more sensitive than mouse toxicity assay in detecting the presence of epsilon-antigen. However, very low titres were detected in gut contents from normal sheep which meant that in a diagnostic situation interpretation of RPHA titres would be difficult. No epsilon-antigen was detected by SRID in gut contents from normal sheep or in gut contents from freshly dead infected sheep. The SRID assay could detect epsilon-antigen in gut contents from infected sheep allowed to decompose for 20 h post-mortem.
开发了两种体外免疫测定法用于检测产气荚膜梭菌D型的ε抗原。发现反向被动血凝试验（RPHA）能够检测低至6×10-7mg/ml的ε抗原浓度，而单向放射免疫扩散技术（SRID）能够检测高于0.01mg/ml的ε抗原浓度。当应用于刚死亡的感染绵羊的肠道内容物时，发现RPHA试验在检测ε抗原的存在方面比小鼠毒性试验更敏感。然而，在正常绵羊的肠道内容物中检测到的滴度非常低，这意味着在诊断情况下，对RPHA滴度的解释会很困难。在正常绵羊的肠道内容物或刚死亡的感染绵羊的肠道内容物中，SRID均未检测到ε抗原。SRID测定法可以在死后分解20小时的感染绵羊的肠道内容物中检测到ε抗原。
