Instituto de Investigaciones Biológicas, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.
Plant Signal Behav. 2012 Feb;7(2):188-92. doi: 10.4161/psb.18843. Epub 2012 Feb 1.
Transcriptional co-activators of the multiprotein bridging factor 1 (MBF1) controls gene expression by connecting transcription factors and the basal transcription machinery. In Arabidopsis thaliana functions of MBF1 genes have been related to stress tolerance and developmental alterations. Endogenous ABA plays a major role in the regulation of Arabidopsis seed dormancy and germination. Seed dormancy and ABA sensitivity are enhanced in ethylene insensitive mutants suggesting that ethylene signal transduction pathway is necessary to fully develop ABA-dependent germination. In this report we showed that a triple knock-down mutant for Arabidopsis MBF1 genes (abc-) has enhanced seed dormancy and displays hypersensitivity to exogenous ABA. In addition, higher ABA contents were detected in abc- seeds after imbibition. These evidences suggest a negative role of MBF1s genes in ABA-dependent inhibition of germination. The participation of MBF1s in ethylene signal transduction pathway is also discussed.
多蛋白桥连因子 1(MBF1)的转录共激活因子通过连接转录因子和基础转录机制来控制基因表达。在拟南芥中,MBF1 基因的功能与胁迫耐受性和发育改变有关。内源性 ABA 在调节拟南芥种子休眠和萌发中起着主要作用。乙烯不敏感突变体中种子休眠和 ABA 敏感性增强,表明乙烯信号转导途径对于充分发育 ABA 依赖性萌发是必需的。在本报告中,我们表明,拟南芥 MBF1 基因的三重敲低突变体(abc-)具有增强的种子休眠性,并对外源 ABA 表现出超敏性。此外,在吸水后 abc-种子中检测到更高的 ABA 含量。这些证据表明 MBF1s 基因在 ABA 依赖性抑制萌发中起负调控作用。还讨论了 MBF1s 在乙烯信号转导途径中的参与。