Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
J Assist Reprod Genet. 2012 Apr;29(4):313-9. doi: 10.1007/s10815-012-9712-3. Epub 2012 Feb 22.
To investigate the effect of human cumulus cells on the maturation and developmental potential of immature oocytes in ICSI cycles.
Immature oocytes were randomly divided into two groups: the cumulus-denuded oocyte group (group A) and the cumulus-intact oocyte group (group B). Only oocytes that reached metaphase II (MII) stage after in vitro maturation were used in the ICSI procedure. In vivo mature sibling MII oocytes served as the control group. Maturation rate, fertilization rate, embryo quality and developmental potential were examined.
There was no significant difference in maturation rate between group A (68.16%) and group B (70.49%; P > 0.05). The total fertilization rate among the three groups was comparable (P > 0.05), while the zygotes with two pronuclei in group A (74.59%) or group B (75.97%) were significantly lower than those in control group (84.29%; P < 0.05). The available embryo rate in group A (11.49%) was markedly lower than that in group B (27.66%; P < 0.05), and both of them were significantly lower than that in control group (62.38%; P < 0.05). The proportion of ≥6-cell embryos in group B (45.74%) was notably higher than in group A (26.44%; P < 0.05), and both were markedly lower than in control group (65.92%; P < 0.05). The proportion of embryos with <10% fragmentation in group A (13.79%) was significantly lower than in group B (29.79%; P < 0.05), and both were notably lower than in control group (42.98%; P < 0.05).
The presence of cumulus cells surrounding the immature oocytes during IVM before ICSI had no influence on nuclear maturation and fertilization, but leads to better subsequent embryonic development. This is perhaps mediated by an improvement in cytoplasmic maturation.
探讨人类卵丘细胞对卵胞浆内单精子注射（ICSI）周期中未成熟卵母细胞成熟和发育潜能的影响。
将未成熟卵母细胞随机分为两组：卵丘细胞去除组（A 组）和卵丘细胞完整组（B 组）。仅将体外成熟后达到中期 II（MII）阶段的卵母细胞用于 ICSI 程序。将体内成熟的姐妹 MII 卵母细胞作为对照组。检查卵母细胞成熟率、受精率、胚胎质量和发育潜能。
A 组（68.16%）和 B 组（70.49%）的卵母细胞成熟率无显著差异（P>0.05）。三组的总受精率相当（P>0.05），但 A 组（74.59%）或 B 组（75.97%）的双核受精卵母细胞明显低于对照组（84.29%；P<0.05）。A 组（11.49%）的可利用胚胎率明显低于 B 组（27.66%；P<0.05），均明显低于对照组（62.38%；P<0.05）。B 组（45.74%）的≥6 细胞胚胎比例明显高于 A 组（26.44%；P<0.05），均明显低于对照组（65.92%；P<0.05）。A 组（13.79%）的碎片<10%的胚胎比例明显低于 B 组（29.79%；P<0.05），均明显低于对照组（42.98%；P<0.05）。
在 ICSI 之前的 IVM 中，卵丘细胞围绕未成熟卵母细胞的存在对核成熟和受精没有影响，但导致随后的胚胎发育更好。这可能是通过细胞质成熟的改善来介导的。
