Department of Chemistry and Center for Innovation in Chemisty, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Luminescence. 2013 Jan-Feb;28(1):76-83. doi: 10.1002/bio.2348. Epub 2012 Feb 21.
A new screening method for flunitrazepam in colourless alcoholic beverages based on a spectroscopic technique is proposed. Absorption and steady-state fluorescence of flunitrazepam and its protonated form with various acids were investigated. The redshift of the wavelength of maximum absorption was distinctively observed in protonated flunitrazepam. An emissive fluorescence at 472 nm was detected in colourless spirits (vodka and tequila) at room temperature. 2-M perchloric acid was the most appropriated proton source. By using electron ionization mass spectrometry and time-dependent density functional theory calculations, the possible structure of protonated flunitrazepam was identified to be 2-nitro-N-methylacridone, an acridone derivative as opposed to 2-methylamino-5-nitro-2'-fluorobenzophenone, a benzophenone derivative.
本文提出了一种基于光谱技术的无色酒精饮料中氟硝西泮的新型筛选方法。研究了氟硝西泮及其与各种酸形成的质子化形式的吸收和稳态荧光。在质子化的氟硝西泮中，观察到最大吸收波长的红移现象。在室温下的无色烈酒（伏特加和龙舌兰酒）中检测到 472nm 的发射荧光。2- 高氯酸是最合适的质子源。通过使用电子电离质谱和时变密度泛函理论计算，确定质子化氟硝西泮的可能结构为 2-硝基-N-甲基吖啶酮，一种吖啶酮衍生物，而不是苯并二酮衍生物 2-甲基氨基-5-硝基-2'-氟苯并酮。
