D'Aloise Paul, Chen Hao
Department of Chemistry and Biochemistry, Ohio University, Athens, OH, USA.
Sci Justice. 2012 Mar;52(1):2-8. doi: 10.1016/j.scijus.2011.08.007. Epub 2011 Sep 15.
Desorption electrospray ionization-mass spectrometry (DESI-MS), a novel ambient ionization technique, was used in this study for determining flunitrazepam in various alcoholic beverages. Using this technique, no pretreatment of the samples was necessary and identification of the drug was accomplished in individual samples in minutes. In addition, the acquired mass spectra provide the information of the identity of the drink based on the detected characteristic ions from the matrices. This study also demonstrates the capability of DESI-MS to perform quantitative analysis of simulated evidence samples with a limit of quantification of 3μg/mL. Furthermore it has been shown that this method can be used for high-throughput analysis whereby six samples were analyzed in a row within 6 minutes and no observable sample carry-over was noted. DESI-MS shows potential as a rapid, sensitive, and selective technique for forensic analysis of spiked beverages which are typical evidence of drug facilitated sexual assault and robbery cases.
解吸电喷雾电离质谱法（DESI-MS）是一种新型的常压电离技术，本研究用其测定各种酒精饮料中的氟硝西泮。使用该技术时，无需对样品进行预处理，数分钟内即可完成单个样品中药物的鉴定。此外，所获得的质谱图基于从基质中检测到的特征离子提供饮料成分的信息。本研究还证明了DESI-MS对模拟物证样品进行定量分析的能力，定量限为3μg/mL。此外，已经表明该方法可用于高通量分析，即在6分钟内连续分析6个样品，且未观察到明显的样品残留。DESI-MS作为一种快速、灵敏且具有选择性的技术，在对掺入药物的饮料进行法医分析方面显示出潜力，此类饮料是药物辅助性性侵和抢劫案件的典型证据。
