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体外精子与人类输卵管细胞的超微结构相互作用

Ultrastructural interaction between spermatozoon and human oviductal cells in vitro.

作者信息

Vigil Pilar, Salgado Ana María, Cortés Manuel E

机构信息

Programa Teen STAR de Educación en Afectividad y Sexualidad, Pontificia Universidad Católica de Chile, Alameda B. O'Higgins 340, Santiago, Chile.

出版信息

J Electron Microsc (Tokyo). 2012 Apr;61(2):123-6. doi: 10.1093/jmicro/dfs002. Epub 2012 Feb 20.

DOI:10.1093/jmicro/dfs002
PMID:22355149
Abstract

The oviduct is an important organ for successful mammalian reproduction. In this work, human oviducts were inseminated and their explants analyzed using scanning electron microscopy in order to study, at a finer ultrastructual level, the interaction between spermatozoon and oviduct in vitro. Results show unequivocally a spermatozoon tightly attached through the acrosomal region of its head to several cilia of the human tubal epithelial cells. This finding proves that spermatozoa do indeed adhere to the endosalpinx, a fact of utmost relevance for the physiology of the reproductive process, since it supports the idea of a spermatozoa reservoir being formed in the oviduct, which is also briefly discussed.

摘要

输卵管是哺乳动物成功繁殖的重要器官。在这项研究中,对人类输卵管进行授精,并使用扫描电子显微镜对其外植体进行分析,以便在更精细的超微结构水平上研究精子与输卵管在体外的相互作用。结果明确显示,精子通过其头部的顶体区域紧密附着在人输卵管上皮细胞的几根纤毛上。这一发现确凿地证明精子确实会附着在输卵管内膜上,这一事实对于生殖过程的生理学极为重要,因为它支持了在输卵管中形成精子库的观点,对此也进行了简要讨论。

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