Pacey A A, Hill C J, Scudamore I W, Warren M A, Barratt C L, Cooke I D
University Department of Obstetrics and Gynaecology, Jessop Hospital for Women, Sheffield, UK.
Hum Reprod. 1995 Feb;10(2):360-6. doi: 10.1093/oxfordjournals.humrep.a135943.
The physical interaction between human spermatozoa and the epithelium of the human uterine (Fallopian) tube was investigated in vitro using a variety of techniques. The 'live' observation of human spermatozoa incubated with 1 day old cultures of tubal epithelium demonstrated that spermatozoa can show a strong physical interaction with epithelial cells; contact with the epithelium appeared to be random and there was no evidence of any taxis toward epithelial cells. The physical interaction (or 'binding') was resistant to gentle washing and was maintained following the addition of glutaraldehyde fixative. The intimate nature of the interaction was confirmed ultrastructurally where both spermatozoa and epithelial membranes were observed to be in close apposition. These results are the first descriptions of sperm-epithelial 'binding' in the human. They are similar to other observations made in a variety of non-human mammalian species. It is suggested that this interaction may be an important feature of normal sperm transport in the human uterine tube in vivo.
利用多种技术在体外研究了人类精子与人体输卵管上皮之间的物理相互作用。对与培养1天的输卵管上皮共同孵育的人类精子进行“活体”观察表明，精子可与上皮细胞表现出强烈的物理相互作用；与上皮的接触似乎是随机的，没有证据表明精子存在趋向于上皮细胞的任何趋化性。这种物理相互作用（或“结合”）能抵抗轻柔冲洗，在添加戊二醛固定剂后仍能保持。超微结构证实了这种相互作用的紧密性质，观察到精子和上皮细胞膜紧密并列。这些结果是对人类精子与上皮“结合”的首次描述。它们与在多种非人类哺乳动物物种中所做的其他观察结果相似。有人提出，这种相互作用可能是人类输卵管内正常精子运输的一个重要特征。
