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可溶性血清 CD81 在慢性丙型肝炎患者中升高，并与丙氨酸氨基转移酶血清活性相关。

Soluble serum CD81 is elevated in patients with chronic hepatitis C and correlates with alanine aminotransferase serum activity.

机构信息

Medizinische Klinik 1, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, Frankfurt am Main, Germany.

出版信息

PLoS One. 2012;7(2):e30796. doi: 10.1371/journal.pone.0030796. Epub 2012 Feb 15.

DOI:10.1371/journal.pone.0030796
PMID:22355327
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3280260/
Abstract

AIM

Cellular CD81 is a well characterized hepatitis C virus (HCV) entry factor, while the relevance of soluble exosomal CD81 in HCV pathogenesis is poorly defined. We performed a case-control study to investigate whether soluble CD81 in the exosomal serum fraction is associated with HCV replication and inflammatory activity.

PATIENTS AND METHODS

Four cohorts were investigated, patients with chronic hepatitis C (n = 37), patients with chronic HCV infection and persistently normal ALT levels (n = 24), patients with long term sustained virologic response (SVR, n = 7), and healthy volunteers (n = 23). Concentration of soluble CD81 was assessed semi-quantitatively after differential centrifugation ranging from 200 g to 100,000 g in the fifth centrifugation fraction by immunoblotting and densitometry.

RESULTS

Soluble CD81 was increased in patients with chronic hepatitis C compared to healthy subjects (p = 0.03) and cured patients (p = 0.017). Patients with chronic HCV infection and persistently normal ALT levels and patients with long term SVR had similar soluble CD81 levels as healthy controls (p>0.2). Overall, soluble CD81 levels were associated with ALT levels (r = 0.334, p = 0.016) and severe liver fibrosis (p = 0.027).

CONCLUSION

CD81 is increased in the exosomal serum fraction in patients with chronic hepatitis C and appears to be associated with inflammatory activity and severity of fibrosis.

摘要

目的

细胞 CD81 是一种经过充分研究的丙型肝炎病毒（HCV）进入因子，而可溶性外泌体 CD81 在 HCV 发病机制中的相关性尚未明确。我们进行了一项病例对照研究，旨在调查外泌体血清部分中的可溶性 CD81 是否与 HCV 复制和炎症活动有关。

患者与方法

我们调查了四个队列，慢性丙型肝炎患者（n=37）、慢性 HCV 感染且持续正常 ALT 水平的患者（n=24）、长期持续病毒学应答（SVR，n=7）和健康志愿者（n=23）。通过免疫印迹和密度计量法，在第五次离心部分中，对 200 g 至 100,000 g 之间的差异离心后的血清外泌体部分中的可溶性 CD81 进行半定量评估。

结果

与健康受试者（p=0.03）和治愈患者（p=0.017）相比，慢性丙型肝炎患者的可溶性 CD81 升高。慢性 HCV 感染且持续正常 ALT 水平的患者和长期 SVR 患者的可溶性 CD81 水平与健康对照组相似（p>0.2）。总体而言，可溶性 CD81 水平与 ALT 水平（r=0.334，p=0.016）和严重肝纤维化（p=0.027）相关。

结论

慢性丙型肝炎患者血清外泌体部分中的 CD81 增加，似乎与炎症活动和纤维化的严重程度有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/785b90ba0fbf/pone.0030796.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/ad1aff8630e9/pone.0030796.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/a71d2748db2d/pone.0030796.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/7400dbab139f/pone.0030796.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/785b90ba0fbf/pone.0030796.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/ad1aff8630e9/pone.0030796.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/a71d2748db2d/pone.0030796.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/7400dbab139f/pone.0030796.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5aa1/3280260/785b90ba0fbf/pone.0030796.g004.jpg

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