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Cdc14b 调控哺乳动物 RNA 聚合酶 II 并抑制细胞周期转录。

Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription.

机构信息

Cell Division and Cancer Group, Spanish National Cancer Research Center (CNIO), E-28029 Madrid, Spain.

出版信息

Sci Rep. 2011;1:189. doi: 10.1038/srep00189. Epub 2011 Dec 12.

DOI:10.1038/srep00189
PMID:22355704
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3240995/
Abstract

Cdc14 is an essential phosphatase in yeast but its role in the mammalian cell cycle remains obscure. We report here that Cdc14b-knockout cells display unscheduled induction of multiple cell cycle regulators resulting in early entry into DNA replication and mitosis from quiescence. Cdc14b dephosphorylates Ser5 at the C-terminal domain (CTD) of RNA polymerase II, a major substrate of cyclin-dependent kinases. Lack of Cdc14b results in increased CTD-Ser5 phosphorylation, epigenetic modifications that mark active chromatin, and transcriptional induction of cell cycle regulators. These data suggest a function for mammalian Cdc14 phosphatases in the control of transcription during the cell cycle.

摘要

Cdc14 是酵母中一种必不可少的磷酸酶，但它在哺乳动物细胞周期中的作用仍不清楚。我们在这里报告说，Cdc14b 敲除细胞显示出多个细胞周期调节剂的非计划性诱导，导致从静止期早期进入 DNA 复制和有丝分裂。Cdc14b 去磷酸化 RNA 聚合酶 II C 端结构域 (CTD) 上的 Ser5，这是细胞周期依赖性激酶的主要底物。缺乏 Cdc14b 会导致 CTD-Ser5 磷酸化增加、标记活性染色质的表观遗传修饰以及细胞周期调节剂的转录诱导。这些数据表明，哺乳动物 Cdc14 磷酸酶在细胞周期中控制转录方面具有功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/33e52c92e331/srep00189-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/cee0872a7aeb/srep00189-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/07bda2a2a4d3/srep00189-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/85d5b242d9e0/srep00189-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/f5faf573afa5/srep00189-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/33e52c92e331/srep00189-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/cee0872a7aeb/srep00189-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/07bda2a2a4d3/srep00189-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/85d5b242d9e0/srep00189-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/f5faf573afa5/srep00189-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f7a/3240995/33e52c92e331/srep00189-f5.jpg

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