丙型肝炎患者肝活检中丙型肝炎病毒（HCV）的免疫组织化学检测。

Immunohistochemical detection of hepatitis C virus (HCV) in liver biopsies of hepatitis C patients.

作者信息

Syed Shuja Izhar, Sadiq Saleem

机构信息

Department of Pathology, Baqai Medical University, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Center, Karachi.

出版信息

J Pak Med Assoc. 2011 Dec;61(12):1198-201.

PMID:22355966

Abstract

OBJECTIVE

Immunohistochemical detection of Hepatitis C virus (HCV) in liver biopsies of hepatitis C patients and to find its association with the histological parameters of grading and staging.

METHODS

This was a prospective study based on liver biopsies of hepatitis C patients. Haematoxylin and Eosin (H and E) stained slides were examined to determine the histological activity, and fibrosis score. The HCV NS3 antigen was detected with Immunohistochemical technique using monoclonal antibody against HCV NS3 protein.

RESULTS

Cases which had a positive immunoreactivity for HCV- NS3 were 47 (94%). A total of 29 (58%) cases had shown grade 3 positivity for HCV-NS3 immunostaining; out of which, 16 cases had grade 3 necroinflammatory activity and 8 cases had fibrosis to the extent of cirrhosis (P<0.05). Out of the total 3 (6%) cases with negative immunoreactivity for HCV-NS3, 2 cases had grade 1 activity and all of these 3 patients had minimal fibrosis amounting to stage 1 (P<0.05).

CONCLUSION

There is a positive association between HCV immunopositivity and the histological parameters of grading and staging, suggesting that greater amounts of virus are present in more advanced chronic liver disease.

摘要

目的

对丙型肝炎患者肝活检组织进行丙型肝炎病毒（HCV）的免疫组织化学检测，并探讨其与分级和分期的组织学参数之间的关联。

方法

这是一项基于丙型肝炎患者肝活检的前瞻性研究。检查苏木精和伊红（H&E）染色切片以确定组织学活性和纤维化评分。使用抗HCV NS3蛋白的单克隆抗体，通过免疫组织化学技术检测HCV NS3抗原。

结果

HCV-NS3免疫反应阳性的病例有47例（94%）。共有29例（58%）病例HCV-NS3免疫染色呈3级阳性；其中，16例有3级坏死性炎症活动，8例纤维化程度达肝硬化（P<0.05）。在HCV-NS对3免疫反应阴性的3例（6%）病例中，2例有1级活动，所有这3例患者纤维化程度均为1期（P<0.05）。