Zhao X, Yang D, Li L
Clinical Immunology Research Unit, Tongji Hospital, Tongji Medical University, Wuhan.
Zhonghua Nei Ke Za Zhi. 1995 Nov;34(11):728-30.
Hepatitis C virus antigen (HCV Ag) and HCV-NS3 protein in paraffin-embedded liver tissue of patients with HCV infection were detected with immunohistochemical method by using polyclonal anti-HCV and monoclonal anti-HCV-NS3 for studying the expression of HCV in liver tissue. The results showed that the detection rate of HCV Ag and HCV-NS3 were 38.3% and 30.0% respectively. All the specimens with HCV-NS3 positive tissue were also those with HCV Ag positive. The stained substance located in the cytoplasm of liver cell, and the stained cells distributed scattered, clustered or diffuse in liver lobular. No obvious infiltration or necrosis was observed around the positive cells. There was obvious difference of the detection rate between different models of serum HCV markers. These results provided some help in understanding the pathogenesis of HCV.
采用多克隆抗丙型肝炎病毒（HCV）和单克隆抗HCV非结构蛋白3（NS3），通过免疫组化方法检测丙型肝炎病毒感染患者石蜡包埋肝组织中的HCV抗原（HCV Ag）和HCV-NS3蛋白，以研究HCV在肝组织中的表达情况。结果显示，HCV Ag和HCV-NS3的检出率分别为38.3%和30.0%。所有HCV-NS3阳性组织的标本同时也是HCV Ag阳性标本。染色物质位于肝细胞的细胞质中，染色细胞在肝小叶中呈散在、聚集或弥漫分布。阳性细胞周围未观察到明显的浸润或坏死。不同模式的血清HCV标志物之间的检出率存在明显差异。这些结果为了解HCV的发病机制提供了一定帮助。
