慢性丙型肝炎中的纤维化：免疫组织化学评估的病毒载量与脂肪变性及细胞铁含量之间的相关性

Fibrosis in Chronic Hepatitis C: Correlation between Immunohistochemically-Assessed Virus Load with Steatosis and Cellular Iron Content.

作者信息

Akl Maha, Hindawi Ali El, Mosaad Maha, Montasser Ahmed, Ray Ahmed El, Khalil Heba, Anas Amgad, Atta Raffat, Paradis Valerie, Hadi Ahmed Abdel, Hammam Olfat

机构信息

Department of Pathology, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt.

Department of Pathology, Faculty of Medicine Cairo University, Cairo, Egypt.

出版信息

Open Access Maced J Med Sci. 2016 Dec 15;4(4):578-584. doi: 10.3889/oamjms.2016.122. Epub 2016 Nov 15.

DOI:10.3889/oamjms.2016.122

PMID:28028394

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5175502/

Abstract

AIM

We aimed study impact of hepatocytic viral load, steatosis, and iron load on fibrosis in chronic hepatitis C and role of VEGF and VEGFR overexpression in cirrhotic cases in evolving HCC.

MATERIAL AND METHODS

Total of 120 cases were included from TBRI and Beaujon Hospital as chronic hepatitis C (CHC), post-hepatitis C cirrhosis, and HCC. Cases of CHC were stained for Sirius red, Prussian blue and immunohistochemically (IHC) for HCV-NS3/NS4. HCC were stained IHC for VEGF and by FISH.

RESULTS

Stage of fibrosis was significantly correlated with inflammation in CHC (P < 0.01). Noticed iron load did not correlate with fibrosis. Steatosis was associated with higher inflammation and fibrosis. The cellular viral load did not correlate with inflammation, steatosis or fibrosis. VEGF by IHC was significantly higher in cases of HCC when compared to cirrhotic group (P < 0.001). Amplification of VEGFR2 was confirmed in 40% of cases of HCC. Scoring of VEGF by IHC was the good indicator of VEGFR2 amplification by FISH (P < 0.005).

CONCLUSION

Grade of inflammation is the factor affecting fibrosis in CHC. The degree of liver damage is not related to cellular viral load or iron load. Steatosis is associated with higher inflammation and fibrosis. VEGF by IHC is correlated with overexpression of VEGFR2 by FISH.

摘要

目的

我们旨在研究慢性丙型肝炎中肝细胞病毒载量、脂肪变性和铁负荷对纤维化的影响，以及血管内皮生长因子（VEGF）和血管内皮生长因子受体（VEGFR）过表达在肝硬化病例进展为肝细胞癌（HCC）中的作用。

材料与方法

从TBRI医院和博若莱医院纳入120例慢性丙型肝炎（CHC）、丙型肝炎后肝硬化和HCC患者。CHC病例进行天狼星红、普鲁士蓝染色及HCV-NS3/NS4免疫组化（IHC）染色。HCC病例进行VEGF免疫组化染色及荧光原位杂交（FISH）检测。

结果

CHC中纤维化分期与炎症显著相关（P < 0.01）。观察到的铁负荷与纤维化无关。脂肪变性与更高程度的炎症和纤维化相关。细胞病毒载量与炎症、脂肪变性或纤维化均无相关性。与肝硬化组相比，HCC病例中VEGF免疫组化表达显著更高（P < 0.001）。40%的HCC病例证实有VEGFR2扩增。VEGF免疫组化评分是FISH检测VEGFR2扩增的良好指标（P < 0.005）。