Drug Metabolism and Toxicology Research Laboratories, Department of Biochemistry, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Andrologia. 2012 Aug;44(4):273-84. doi: 10.1111/j.1439-0272.2012.01279.x. Epub 2012 Feb 22.
This study evaluated the effects of kolaviron, a biflavonoid from Garcinia kola seed, and quercetin on cadmium-induced reproductive toxicity in rats. Adult male rats were administered with either cadmium (15 mg kg(-1)) alone or in combination with kolaviron (200 mg kg(-1)) or quercetin (10 mg kg(-1)) daily for 5 days. Cadmium-treated rats showed (P < 0.05) decrease in the body weight gain, testis and epididymis weights. However, upon co-administration of kolaviron or quercetin, these changes were significantly reversed in cadmium-treated rats. Also, administration of kolaviron or quercetin significantly prevented cadmium-mediated decrease in sperm motility and epididymal sperm concentration and reversed the increased level of sperm abnormality to near control. In testes and sperm, cadmium treatment resulted in significant decrease in the activities of superoxide dismutase, catalase and glutathione peroxidase, whereas it increased glutathione S-transferase activity as well as hydrogen peroxide and malondialdehyde levels. While plasma levels of triiodothyronine and tetraiodothyronine remained unaffected, the levels of testosterone, luteinising hormone and follicle stimulating hormone were decreased in cadmium-treated rats. Cadmium treatment caused mild congestion of interstitial vessels and oedema in the testes. Taken together, kolaviron and quercetin inhibited the adverse effects of cadmium on the antioxidant enzymes, markers of oxidative stress, endocrine and testicular structure in rats.
本研究评估了来自可乐果种子的双黄酮 kolaviron 和槲皮素对镉诱导的大鼠生殖毒性的影响。成年雄性大鼠每天给予镉（15 mg/kg）单独或与 kolaviron（200 mg/kg）或槲皮素（10 mg/kg）联合给药 5 天。镉处理的大鼠体重增加、睾丸和附睾重量下降（P<0.05）。然而，当 kolaviron 或槲皮素共同给药时，这些变化在镉处理的大鼠中显著逆转。此外，kolaviron 或槲皮素的给药显著防止了镉介导的精子活力和附睾精子浓度下降，并将精子畸形水平升高逆转至接近对照。在睾丸和精子中，镉处理导致超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性显著下降，而谷胱甘肽 S-转移酶活性以及过氧化氢和丙二醛水平增加。虽然三碘甲状腺原氨酸和四碘甲状腺原氨酸的血浆水平保持不变，但镉处理的大鼠的睾酮、促黄体生成素和卵泡刺激素水平降低。镉处理导致睾丸间质血管轻度充血和水肿。综上所述，kolaviron 和槲皮素抑制了镉对大鼠抗氧化酶、氧化应激标志物、内分泌和睾丸结构的不良影响。
