Veterinary and Animal Research Centre (CECAV) and Veterinary Science Department, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal.
Foodborne Pathog Dis. 2012 Apr;9(4):338-45. doi: 10.1089/fpd.2011.1035. Epub 2012 Feb 22.
The aim of this study was to evaluate the effects of various glucosinolate-derived hydrolysis products (HP) as antibacterial compounds against Enterobacteriaceae and Enterococcaceae isolated from intestinal segments of healthy pigs collected directly from slaughter-houses in the North of Portugal. Using a previously described disk-diffusion bioassay, all HP were tested at six different doses (0.015, 0.15, 0.75, 1.5, 3.0, and 15.0 μmoles) in dimethyl-sulfoxide (DMSO), with the exception of sulforaphane (SFN), which was not tested at 15.0 μmoles. Positive (antibiotic standard) and negative controls (DMSO) were included in all experiments. All the experiments were conducted in triplicate. In vitro inhibition of the bacterial growth by the HP was proportional to the concentration used and in many cases was even higher than for the gentamycin, the antibiotic control. The results clearly showed that the glucosinolates-derived HPs were very effective in vitro inhibitors of bacterial growth. The natural products, and specifically the isothiocyanates, should be evaluated as potential alternative control agents for potentially pathogenic bacteria (e.g., dietary amendment of pig foods with glucosinolate-containing plants).
本研究旨在评估各种源自硫代葡萄糖苷的水解产物（HP）作为抗菌化合物对从葡萄牙北部屠宰场直接采集的健康猪肠道段分离出的肠杆菌科和肠球菌科的影响。使用先前描述的圆盘扩散生物测定法，在二甲基亚砜（DMSO）中以六种不同剂量（0.015、0.15、0.75、1.5、3.0 和 15.0 μmoles）测试所有 HP，除了萝卜硫素（SFN），因为它未在 15.0 μmoles 下进行测试。所有实验均包含阳性（抗生素标准）和阴性对照（DMSO）。所有实验均重复进行三次。HP 对细菌生长的体外抑制作用与使用的浓度成正比，在许多情况下甚至高于抗生素对照的庆大霉素。结果清楚地表明，源自硫代葡萄糖苷的 HP 是非常有效的细菌生长体外抑制剂。天然产物，特别是异硫氰酸盐，应作为潜在的病原菌（例如，在含有硫代葡萄糖苷的植物的猪饲料中添加饮食）的潜在替代控制剂进行评估。
