CITAB-Centre for the Research and Technology for Agro-Environment and Biological Sciences, Universidade de Trásos-Montes e Alto Douro, Apartado 1013, 5000-801 Vila Real, Portugal.
Med Chem. 2013 Sep;9(6):861-6. doi: 10.2174/1573406411309060010.
With this study we intend to evaluate the effects of 8 different glucosinolate hydrolysis products including isothiocyanates as antibacterial compounds against Aeromonadaceae, isolated from intestinal segments of pigs collected directly from slaughter-houses in the North of Portugal. Four Aeromonas species, A. allosaccharophila, A. hydrophila, A. media and A. veronii were identified. Using disc-diffusion bioassay all compounds were tested at 6 different doses (0.015, 0.15, 0.75, 1.5 and 3.0 µmoles) in dimethyl-sulfoxide (DMSO). Antibiotic standard (gentamicin) and DMSO (negative control) were included in all experiments. Minimum and maximum dose inhibitions (in µmoles) were assessed. To our knowledge, this is the first study of antimicrobial activity of glucosinolate hydrolysis products against Aeromonas species. The results showed that glucosinolate hydrolysis products and particularly the isothiocyanates have antimicrobial activity, which was proportional to the concentration used. However, not all revealed the same tendency, which means that the chemical structure of each compound is fundamental to understand their effectiveness. Among the different isothiocyanates the benzylisothiocyanate, sulforaphane and 2-phenylethylisothiocyanate were the most effective in vitro inhibitors of bacterial growth. This in vitro study provides enough data to demonstrate the potential use of these natural dietary chemicals for treating infectious diseases caused by Aeromonas spp.
本研究旨在评估 8 种不同的硫代葡萄糖苷水解产物（包括异硫氰酸盐）作为抗菌化合物对从葡萄牙北部屠宰场直接采集的猪肠道段分离出的气单胞菌属的影响。鉴定出 4 种气单胞菌，分别为嗜水气单胞菌、杀鲑气单胞菌、温和气单胞菌和维罗纳气单胞菌。使用圆盘扩散生物测定法，将所有化合物在 6 种不同剂量（0.015、0.15、0.75、1.5 和 3.0 µmoles）的二甲亚砜（DMSO）中进行测试。所有实验均包含抗生素标准（庆大霉素）和 DMSO（阴性对照）。评估最小和最大剂量抑制（µmoles）。据我们所知，这是首次研究硫代葡萄糖苷水解产物对气单胞菌属的抗菌活性。结果表明，硫代葡萄糖苷水解产物，特别是异硫氰酸盐具有抗菌活性，其活性与使用的浓度成正比。然而，并非所有产物都表现出相同的趋势，这意味着每个化合物的化学结构对于理解其有效性至关重要。在不同的异硫氰酸盐中，苄基异硫氰酸盐、萝卜硫素和 2-苯乙基异硫氰酸盐是体外抑制细菌生长最有效的抑制剂。这项体外研究提供了足够的数据，证明了这些天然饮食化学物质在治疗由气单胞菌属引起的感染性疾病方面的潜在用途。
