Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Department of Botany, Faculty of Science, Cairo University, Giza, Egypt.
BMC Complement Med Ther. 2022 Aug 27;22(1):229. doi: 10.1186/s12906-022-03710-1.
The aim of this study is investigate the antimicrobial effect of plant oils against bacterial strains isolated from neonatal asymptomatic bacteriuria (ABU) and to evaluate the antiseptic effect of the most potent one.
The antimicrobial effect of 17 plant oils were tested against 15- gram-negative bacterial strains recovered from cases of neonatal ABU (11 Escherichia. coli, 3 Klebsiella pneumonia, and 1 Pseudomonas aeruginosa) using the agar well diffusion method. The micro-dilution method was performed to investigate the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) in concentrations ranging from 1.95 μg/ml to 500 μg/ml. The evaluation of the antiseptic activity of the Eruca sativa (arugula) seed oil was investigated using time-kill assay in concentrations ranging from 50 μg/ml to 0.195 μg/ml.
All tested oils showed variable antimicrobial activities against the tested strains. Arugula, wheat germ, cinnamon, parsley, dill, and onion oils were the most active oils. Among them, arugula oil was the most active oil with MIC and MIC were 3.9 μg/ml and 31.3 μg/ml respectively. MBC and MBC of arugula oil were 15.6 μg/ml and 125μg/ml respectively. The time-kill assay of arugula oil indicated that a concentration of 100 μg/ml completely killed nine of the tested strains after 10 min and reduced the CFU/ml of the rest of the strains by 3 log at the same time interval.
Arugula seed oil could be a potentially used as an antiseptic especially for neonates.
本研究旨在探讨植物精油对新生儿无症状菌尿（ABU）分离细菌株的抗菌作用，并评估其中最有效的抗菌作用。
采用琼脂孔扩散法检测 17 种植物精油对 15 株革兰氏阴性菌（11 株大肠埃希菌、3 株肺炎克雷伯菌和 1 株铜绿假单胞菌）的抗菌作用。采用微量稀释法测定 MIC 和 MBC，浓度范围为 1.95μg/ml 至 500μg/ml。采用时间杀菌试验评估艾鲁卡（芝麻菜）籽油在 50μg/ml 至 0.195μg/ml 浓度范围内的杀菌活性。
所有测试的油对测试菌株均显示出不同的抗菌活性。芝麻菜、小麦胚芽、肉桂、欧芹、莳萝和洋葱油是最有效的油。其中，芝麻菜籽油的抗菌作用最强，MIC 和 MIC 分别为 3.9μg/ml 和 31.3μg/ml。芝麻菜籽油的 MBC 和 MBC 分别为 15.6μg/ml 和 125μg/ml。芝麻菜籽油的时间杀菌试验表明，浓度为 100μg/ml 的油在 10 分钟后可完全杀死 9 株受试菌株，同时使其余菌株的 CFU/ml 减少 3 个对数级。
芝麻菜籽油可能是一种潜在的抗菌剂，特别是对新生儿。
