Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang, People's Republic of China.
Acta Histochem. 2012 Dec;114(8):779-84. doi: 10.1016/j.acthis.2012.01.005. Epub 2012 Feb 20.
The objective of the present study was to evaluate the expression of T-box expressed in T cells (T-bet) and GATA binding protein 3 (GATA-3) in the eutopic endometrium from women with endometriosis. Endometrial tissues were collected from 20 women with laparoscopically confirmed endometriosis and 20 women without endometriosis. T-bet and GATA-3 expression was measured by quantitative real-time PCR (qPCR), immunohistochemistry and Western blot analysis. Eutopic endometrial tissues from patients with endometriosis expressed lower levels of T-bet mRNA and high levels of GATA-3 mRNA, leading to a significant lower T-bet/GATA-3 mRNA ratio (P<0.05). Western blot analysis showed that the T-bet/GATA-3 protein ratio in endometriosis group was also statistically lower than that in the control group (P<0.05). These results suggested that T-bet and GATA-3 may act as cytokine regulatory genes, and the Th2-specific transcription factor, GATA-3, probably plays an essential role in the immune response and the development of endometriosis.
本研究旨在评估 T 细胞表达的 T 盒转录因子（T-bet）和 GATA 结合蛋白 3（GATA-3）在子宫内膜异位症患者在位子宫内膜中的表达。收集了 20 例经腹腔镜确诊为子宫内膜异位症的患者和 20 例无子宫内膜异位症的患者的子宫内膜组织。采用实时定量 PCR（qPCR）、免疫组织化学和 Western blot 分析检测 T-bet 和 GATA-3 的表达。子宫内膜异位症患者的在位子宫内膜组织中 T-bet mRNA 表达水平较低，GATA-3 mRNA 表达水平较高，导致 T-bet/GATA-3 mRNA 比值显著降低（P<0.05）。Western blot 分析显示，子宫内膜异位症组的 T-bet/GATA-3 蛋白比值也明显低于对照组（P<0.05）。这些结果表明，T-bet 和 GATA-3 可能作为细胞因子调节基因发挥作用，Th2 特异性转录因子 GATA-3 可能在免疫反应和子宫内膜异位症的发生发展中发挥重要作用。
