Chen Jian-Ping, Zhao Wan-Ying, He Nian-Hai, He Shui-Xiang, Wang Gang, Wang Wan-Jun
Department of Pediatrics, Southwest Hospital, Third Military Medical University, Chongqing, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2012 Feb;14(2):120-3.
To elucidate whether the polymorphism of asthma immune regulator gene TIM-4 is associated with the risk of childhood allergic asthma in the southwest region of China.
TIM-4 gene promoter region RS6882076 and intron RS4704727 were studied. PCR-RFLP was used to test the genotypes of two polymorphism loci among 579 cases (average 7.2 years old) of asthma and 524 controls (average 7.6 years old) in a case-control study.
There were significant differences in the frequency of gene types at RS4704727 site between the asthma and the control groups (P<0.01). The results of PCR-RFLP showed that the polyporphisms of RS6882076 and RS4704727 in TIM-4 gene were present in this study population. The frequency of T allele at the RS4704727 site in the asthma group was significantly lower than that in the control group (OR=1.603; 95%CI 1.304-1.971; P<0.01). There were no significant differences in the frequencies of gene types and allele at RS6882076 site between the two groups (P>0.05).
RS4704727 polymorphism of TIM-4 gene may be associated with childhood asthma, providing a better understanding of the pathogenesis of childhood asthma in the Southwest region of China.
阐明哮喘免疫调节基因TIM-4的多态性是否与中国西南地区儿童过敏性哮喘的发病风险相关。
研究TIM-4基因启动子区域RS6882076和内含子RS4704727。在一项病例对照研究中，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测579例哮喘患儿（平均7.2岁）和524例对照者（平均7.6岁）中两个多态性位点的基因型。
哮喘组与对照组在RS4704727位点的基因类型频率存在显著差异（P<0.01）。PCR-RFLP结果显示，本研究人群中存在TIM-4基因RS6882076和RS4704727的多态性。哮喘组RS4704727位点T等位基因频率显著低于对照组（OR=1.603；95%CI 1.304-1.971；P<0.01）。两组在RS6882076位点的基因类型和等位基因频率无显著差异（P>0.05）。
TIM-4基因的RS4704727多态性可能与儿童哮喘相关，有助于更好地理解中国西南地区儿童哮喘的发病机制。
