Chen Chan, Wang Zhi-yi, Wang Liang-xing, Du Xiao-hong, Zhao Xiao-wei
Cadres Ward, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang Province 325000, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 Oct;29(10):731-4.
To study the effects of puerarin on proliferation, apoptosis and Kv1.5 gene expression of rat pulmonary artery smooth muscle cells (PASMCs) induced by hypoxia.
The rat PASMCs were divided into 5 groups: control group, hypoxia group, hypoxia plus puerarin (1 × 10(-5) mol/L) group, hypoxia plus puerarin (1 × 10(-4) mol/L) group and hypoxia plus puerarin (1 × 10(-3) mol/L) group, and cultured at 37°C for 24 h. The proliferation of rat PASMCs was detected by CCK-8 assay and flow cytometry, the activity of caspase-3 was measured with spectrophotometric method, Kv1.5 protein was detected by western blot, Kv1.5 mRNA was detected by real-time PCR.
The cell viability and proportion of synthesis phase in control group were 0.940 ± 0.045 and 9.67% ± 1.28%, which were significantly lower than those (1.296 ± 0.034 and 18.19% ± 1.19%) in hypoxia group (P < 0.05). The Caspase-3 activity, Kv 1.5 protein and Kv 1.5 mRNA in control group were 0.1073 ± 0.0113, 0.886 ± 0.038 and 0.0377 ± 0.0031, which were significantly higher than those (0.0664 ± 0.0049, 0.602 ± 0.064 and 0.0108 ± 0.0014) in hypoxia group (P < 0.05). As compared with hypoxia group, the cell viability and proportion of synthesis phase in 3 hypoxia plus puerarin groups significantly decreased, and the Caspase-3 activity, Kv 1.5 protein and Kv 1.5 mRNA in 3 hypoxia plus puerarin groups significantly enhanced (P < 0.05).
Puerarin could decrease the proliferation and increase the apoptosis induced by hypoxia in rat PASMCs, and the up-regulated expression of Kv1.5 gene may be the mechanism of puerarin effects.
研究葛根素对缺氧诱导的大鼠肺动脉平滑肌细胞（PASMCs）增殖、凋亡及Kv1.5基因表达的影响。
将大鼠PASMCs分为5组：对照组、缺氧组、缺氧加葛根素（1×10⁻⁵mol/L）组、缺氧加葛根素（1×10⁻⁴mol/L）组和缺氧加葛根素（1×10⁻³mol/L）组，于37℃培养24小时。采用CCK-8法和流式细胞术检测大鼠PASMCs的增殖情况，用分光光度法测定caspase-3活性，通过蛋白质印迹法检测Kv1.5蛋白，用实时荧光定量PCR检测Kv1.5 mRNA。
对照组细胞活力和合成期比例分别为0.940±0.045和9.67%±1.28%，显著低于缺氧组（1.296±0.034和18.19%±1.19%）（P<0.05）。对照组Caspase-3活性、Kv 1.5蛋白和Kv 1.5 mRNA分别为0.1073±0.0113、0.886±0.038和0.0377±0.0031，显著高于缺氧组（0.0664±0.0049、0.602±0.064和0.0108±0.0014）（P<0.05）。与缺氧组相比，3个缺氧加葛根素组的细胞活力和合成期比例显著降低，3个缺氧加葛根素组的Caspase-3活性、Kv 1.5蛋白和Kv 1.5 mRNA显著增强（P<0.05）。
葛根素可降低缺氧诱导的大鼠PASMCs增殖并增加其凋亡，Kv1.5基因表达上调可能是葛根素发挥作用的机制。
