Zhang Xiaodan, Zhao Fenghua, Zhang Xiumei, Sheng Jiejing
Haerbin University of Commerce, Haerbin 150076, China.
Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(16):2255-8.
To investigate the contribution of mitochondrial pathway in the apoptosis induced by puerarin (PUE) in pulmonary artery smooth muscle cells.
Cultured rat pulmonary artery smooth muscle cells (PASMC) were intervened by high, middle and low dose of puerarin (1.5 x 10(-3), 1.5 x 10(-4), 1.5 x 10(-5) mol x L(-1)). The change of mitochondrial membrane potential was observed. Western blot detected the expression of apoptosis-related gene Caspase-9, Bax and Bcl-2 protein.
Compared with the control group, mitochondrial membrane potential significantly decreased in puerarin groups. Puerarin can enhance the expression of Caspase-9 and Bax protein, decrease the expression of Bcl-2 protein. Puerarin also has a concentration-dependent on the induction of PASMC.
Puerarin can induce PASMC apoptosis through mitochondrial pathway.
探讨葛根素（PUE）诱导肺动脉平滑肌细胞凋亡中线粒体途径的作用。
用高、中、低剂量葛根素（1.5×10⁻³、1.5×10⁻⁴、1.5×10⁻⁵mol·L⁻¹）干预培养的大鼠肺动脉平滑肌细胞（PASMC）。观察线粒体膜电位的变化。蛋白质免疫印迹法检测凋亡相关基因Caspase-9、Bax和Bcl-2蛋白的表达。
与对照组相比，葛根素各剂量组线粒体膜电位均明显降低。葛根素可增强Caspase-9和Bax蛋白的表达，降低Bcl-2蛋白的表达。葛根素对PASMC的诱导作用也具有浓度依赖性。
葛根素可通过线粒体途径诱导PASMC凋亡。
