Mirsky Paul, Chatterjee Anirban, Sauer-Budge Alexis F, Sharon Andre
Fraunhofer Center for Manufacturing Innovation, Brookline, MA 02130, USA.
J Lab Autom. 2012 Apr;17(2):116-24. doi: 10.1177/2211068211424102. Epub 2012 Jan 24.
Sample preparation for DNA and RNA assays is a prime candidate for laboratory automation. A novel, parallel processing device that performs the three separate liquid-handling functions necessary for such sample preparation-dispensing, pipetting, and pressurizing-is presented. The device comprises an array of fine nozzles connected by fluidic channels to automatically and precisely distribute flow between one source and an array of points. The design principles, as well as the experimental and computational methods used to develop the device, are described. Test results, including accuracy, uniformity, volume range, and timing, are presented. The functionality of the device is demonstrated by performing a solid-phase extraction of DNA with two types of microcolumns.
用于DNA和RNA分析的样本制备是实验室自动化的主要候选对象。本文介绍了一种新型的并行处理设备，该设备可执行此类样本制备所需的三项独立液体处理功能——分配、移液和加压。该设备包括由流体通道连接的一系列细喷嘴，以自动且精确地在一个源和一系列点之间分配流体。文中描述了该设备的设计原理以及用于开发该设备的实验和计算方法。还给出了测试结果，包括准确性、均匀性、体积范围和计时。通过使用两种类型的微柱对DNA进行固相萃取，展示了该设备的功能。
