Stangegaard Michael, Meijer Per-Johan, Børsting Claus, Hansen Anders J, Morling Niels
Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
J Lab Autom. 2012 Oct;17(5):378-86. doi: 10.1177/2211068212448427. Epub 2012 May 31.
We have implemented and validated automated protocols for a wide range of processes such as sample preparation, PCR setup, and capillary electrophoresis setup using small, simple, and inexpensive automated liquid handlers. The flexibility and ease of programming enable the Biomek 3000 to be used in many parts of the laboratory process in a modern forensic genetics laboratory with low to medium sample throughput. In conclusion, we demonstrated that sample processing for accredited forensic genetic DNA typing can be implemented on small automated liquid handlers, leading to the reduction of manual work as well as increased quality and throughput.
我们已经使用小型、简单且价格低廉的自动液体处理仪，实现并验证了一系列广泛流程的自动化协议，如样品制备、PCR设置和毛细管电泳设置。编程的灵活性和简便性使Biomek 3000能够在样品通量低至中等的现代法医遗传学实验室的许多实验室流程环节中使用。总之，我们证明了经认可的法医遗传DNA分型的样品处理可以在小型自动液体处理仪上实现，从而减少了人工操作，提高了质量和通量。
