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应用重复 DNA 探针的原位杂交检测间期染色体变异：在细胞遗传学分析和诱变试验中的潜在应用。

Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing.

机构信息

Dipartimento di Genetica e Microbiologia 'A. Buzzati-Traverso', Univ. di Pavia, Via S. Epifanio 14, 27100, Pavia, Italy.

出版信息

Cytotechnology. 1987 Oct;1(1):13-7. doi: 10.1007/BF00351116.

DOI:10.1007/BF00351116
PMID:22358434
Abstract

Individual chromosomes can be identified by means of in situ hybridization with DNA probes for chromosome-specific repetitive sequences. The efficiency and sensitivity of the method are strictly dependent on the characteristics of the probes and the experimental conditions. Using three probes with different copy numbers, we demonstrated that the target chromosomes can be visualized in interphase when the homologous sequences are repeated at least 50 times.Possible applications of interphase analysis to clinical cytogenetics and mutagenicity testing are discussed.

摘要

可以通过用染色体特异性重复序列的 DNA 探针进行原位杂交来鉴定单个染色体。该方法的效率和灵敏度严格取决于探针的特性和实验条件。使用三个具有不同拷贝数的探针，我们证明了当同源序列重复至少 50 次时，靶染色体可以在间期被可视化。讨论了间期分析在临床细胞遗传学和诱变试验中的可能应用。

相似文献

1
Detection of chromosome variation in interphase by in situ hybridization with repetitive DNA probes: potential applications to cytogenetic analysis and mutagenicity testing.应用重复 DNA 探针的原位杂交检测间期染色体变异：在细胞遗传学分析和诱变试验中的潜在应用。
Cytotechnology. 1987 Oct;1(1):13-7. doi: 10.1007/BF00351116.
2
Detection of monosomy in interphase nuclei and identification of marker chromosomes using biotinylated alpha-satellite DNA probes.利用生物素化α-卫星DNA探针检测间期核中的单体性并鉴定标记染色体。
Cancer Genet Cytogenet. 1991 Jan;51(1):23-33. doi: 10.1016/0165-4608(91)90004-e.
3
Flow cytometric quantification of human chromosome specific repetitive DNA sequences by single and bicolor fluorescent in situ hybridization to lymphocyte interphase nuclei.通过对淋巴细胞间期核进行单色和双色荧光原位杂交，采用流式细胞术对人类染色体特异性重复DNA序列进行定量分析。
Cytometry. 1990;11(1):153-64. doi: 10.1002/cyto.990110118.
4
Rapid fluorescence in situ hybridization with repetitive DNA probes: quantification by digital image analysis.使用重复DNA探针的快速荧光原位杂交：通过数字图像分析进行定量
Cytometry. 1994 Sep 1;17(1):13-25. doi: 10.1002/cyto.990170103.
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Interphase cytogenetics of hematological cancer: comparison of classical karyotyping and in situ hybridization using a panel of eleven chromosome specific DNA probes.血液系统恶性肿瘤的间期细胞遗传学：使用一组11种染色体特异性DNA探针比较经典核型分析和原位杂交
Cancer Res. 1991 Apr 1;51(7):1959-67.
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Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84.通过放射性和非放射性原位杂交技术可视化特定靶DNA来检测人类间期核中的染色体畸变：用探针L1.84诊断18三体综合征
Hum Genet. 1986 Dec;74(4):346-52. doi: 10.1007/BF00280484.
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[Cytogenetic study of 121 patients suffering from various hematologic neoplasms using the in situ hybridization technique].[运用原位杂交技术对121例各种血液系统肿瘤患者进行细胞遗传学研究]
Sangre (Barc). 1996 Jun;41(3):201-9.
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An approach for quantitative assessment of fluorescence in situ hybridization (FISH) signals for applied human molecular cytogenetics.一种用于应用人类分子细胞遗传学的荧光原位杂交（FISH）信号定量评估方法。
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DNA in situ hybridization (interphase cytogenetics) versus comparative genomic hybridization (CGH) in human cancer: detection of numerical and structural chromosome aberrations.人类癌症中DNA原位杂交（间期细胞遗传学）与比较基因组杂交（CGH）：检测染色体数目和结构畸变
Acta Histochem. 2000 Feb;102(1):85-94. doi: 10.1078/0065-1281-00540.
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[Interphase cytogenetics--a new technique for analysis of acquired genetic changes in tumor cells].[间期细胞遗传学——一种分析肿瘤细胞获得性基因改变的新技术]
Ugeskr Laeger. 1993 Dec 20;155(51):4158-62.

本文引用的文献

1
Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.通过原位杂交对G带人类染色体单拷贝DNA序列进行定位
Chromosoma. 1981;83(3):431-9. doi: 10.1007/BF00327364.
2
Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry.通过流式细胞术分选后人类X染色体代表性基因组文库的克隆。
Nature. 1981 Oct 1;293(5831):374-6. doi: 10.1038/293374a0.
3
Sex chromosome positions in human interphase nuclei as studied by in situ hybridization with chromosome specific DNA probes.
通过使用染色体特异性DNA探针进行原位杂交研究人类间期核中的性染色体位置。
Hum Genet. 1984;67(3):317-25. doi: 10.1007/BF00291361.
4
Isolation and characterization of an alphoid centromeric repeat family from the human Y chromosome.从人类Y染色体中分离和鉴定一个α卫星着丝粒重复序列家族。
J Mol Biol. 1985 Apr 20;182(4):477-85. doi: 10.1016/0022-2836(85)90234-7.
5
The organization of two related subfamilies of a human tandemly repeated DNA is chromosome specific.人类串联重复DNA的两个相关亚家族的组织具有染色体特异性。
Hum Genet. 1985;70(4):302-10. doi: 10.1007/BF00295365.
6
DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.通过原位杂交将DNA探针定位在18p11.3带，并鉴定出一条小的额外染色体。
Hum Genet. 1985;69(3):268-71. doi: 10.1007/BF00293038.
7
Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84.通过放射性和非放射性原位杂交技术可视化特定靶DNA来检测人类间期核中的染色体畸变：用探针L1.84诊断18三体综合征
Hum Genet. 1986 Dec;74(4):346-52. doi: 10.1007/BF00280484.
8
Genetic analysis of the fragile-X mental retardation syndrome with two flanking polymorphic DNA markers.利用两个侧翼多态性DNA标记对脆性X智力低下综合征进行基因分析。
Proc Natl Acad Sci U S A. 1986 Feb;83(4):1016-20. doi: 10.1073/pnas.83.4.1016.