散在重复 L1 家族在小鼠胸腺发生过程中差异转录。

Interspersed repetitive L1 family is differentially transcribed during the murine thymus ontogeny.

机构信息

Division of Biophysics, CRE ENEA Casaccia, Via Anguillarese 303, 00060, Rome, Italy.

出版信息

Cytotechnology. 1987 Oct;1(1):65-71. doi: 10.1007/BF00351125.

Abstract

Repetitive L1 family in the first LINE (Long Interspersed Element) found in mammals. Structural and evolutionary studies demonstrated the capability of this family to play a functional role and several examples of L1 transcription have been reported in humans and mice especially in tumor cell lines.In this paper a general decrease in the L1 transcription level in different murine thymoma cell lines transformed by different means as compared to a normal counterpart represented by adult thymus is shown. When the same kind of analysis has been performed on normal thymus at different stages of differentiation (17th day embryos, new born, 30th day after birth) a modulated pattern of L1 transcription with a peak in the new born thymus was found. Furthermore, heterogeneous L1 transcription in all the above cases is demonstrated and its possible functional meaning discussed.

摘要

在哺乳动物的第一 LINE（长散布元件）中发现了重复的 L1 家族。结构和进化研究表明，这个家族具有发挥功能作用的能力，并且在人类和小鼠中已经报道了几个 L1 转录的例子，特别是在肿瘤细胞系中。在本文中，与正常对照（由成年胸腺代表）相比，不同方法转化的不同小鼠胸腺瘤细胞系中 L1 转录水平普遍降低。当对不同分化阶段的正常胸腺（17 天胚胎、新生、出生后 30 天）进行相同的分析时，发现 L1 转录呈调制模式，在新生胸腺中达到峰值。此外，在所有上述情况下都证明了 L1 转录的异质性，并讨论了其可能的功能意义。