动物细胞培养的模拟与动态优化。
Simulation and dynamic optimisation of animal cell culture.
机构信息
Dept. of Chemical Engineering, University of Sydney, NSW, 2006, Australia.
出版信息
Cytotechnology. 1997 Jan;23(1-3):13-7. doi: 10.1023/A:1007963501575.
In animal cell culture, there are some 25 substrates that both have a significant effect on the culture performance and which can be measured with relative ease. A detailed dynamic simulation for such a culture has been produced and an optimisation policy that use this model to identify ideal media conditions has been developed. This paper describes an extension of that work to include the dynamic optimisation of cultures under fed-batch operation. Two different types of feeding policy were considered - in the first, discrete shots of feed were supplied, while in the second, feed was added continuously. Both policies offered significant improvements in the predicted productivity of the culture - up to 30% that of an experimentally "optimised"batch culture.
在动物细胞培养中，有大约 25 种基质对培养性能有显著影响，并且可以相对容易地测量。已经对这样的培养进行了详细的动态模拟，并开发了一种优化策略，该策略使用该模型来确定理想的培养基条件。本文描述了将该工作扩展到包括分批补料操作下的培养动态优化。考虑了两种不同类型的补料策略 - 在第一种策略中，供应离散的补料液，而在第二种策略中，连续添加补料。这两种策略都显著提高了培养物的预测生产力 - 比实验“优化”的分批培养提高了 30%。
Zotero 插件