Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, 812, Japan.
Cytotechnology. 1997 Jan;23(1-3):119-25. doi: 10.1023/A:1007971906061.
In today's 'modern' society, no one can escape from the stresses of daily life. Stress stimulates the secretion of stress hormones (e.g. cortisol or noradrenaline) which generally suppress the immune response system, thus rendering the body vulnerable to infectious diseases and cancer. Therefore finding anti-stress food components, which diminish and/or inhibit the stress related suppression of the immune response system would be helpful in maintaining and promoting the health of the human population. Here we established a screening system for anti-stress substances using the cultured human cell line MG-63. The production of interferon-β (IFN-β) by MG-63 cells super-induced by Poly (I): Poly (C) was shown to decrease in a dose dependent manner upon the addition of 0.01-10 μg/ml of cortisol or noradrenaline (NA). 1,2-Diacylglycerol (DG) was demonstrated to abrogate this suppression. Lipid from the fermented milk, kefir, also inhibited the influence of cortisol. Kefiran, a polysaccharide secreted from L. kafiranofasiens GKL-28 diminished the cortisol or NA influenced IFN-β production. But phosphatidylcholine had no significant effect in this system. These results suggest that DG, lipids from kefir and kefiran may be equated as anti-stress food component.Abbreviations DG - diacylglycerol; IFN-β - interferon-β; NA - noradrenaline; PC - phosphatidylcholine; Poly (I):Poly (C) - polyinosinic polycytidylic acid.
在当今的“现代”社会中，没有人能逃避日常生活的压力。压力会刺激应激激素（如皮质醇或去甲肾上腺素）的分泌，这些激素通常会抑制免疫系统，从而使身体容易受到传染病和癌症的侵害。因此，寻找能够减轻和/或抑制与应激相关的免疫系统抑制作用的抗应激食物成分，将有助于维持和促进人类健康。在这里，我们使用培养的人骨肉瘤细胞系 MG-63 建立了一种抗应激物质的筛选系统。用 Poly（I）：Poly（C）超诱导的 MG-63 细胞产生干扰素-β（IFN-β）的能力，随着皮质醇或去甲肾上腺素（NA）添加量从 0.01-10 μg/ml 的剂量依赖性降低。1,2-二酰基甘油（DG）被证明可以消除这种抑制作用。发酵乳酸奶的脂质也抑制了皮质醇的影响。从 L. kafiranofasiens GKL-28 分泌的多糖 kefiran 也减少了皮质醇或 NA 对 IFN-β产生的影响。但卵磷脂在该系统中没有显著影响。这些结果表明，DG、酸奶中的脂质和 kefiran 可以被视为抗应激食物成分。缩写词 DG - 二酰基甘油；IFN-β - 干扰素-β；NA - 去甲肾上腺素；PC - 磷脂酰胆碱；Poly（I）：Poly（C）- 聚肌苷酸：聚胞苷酸。
