Osada K, Nagira K, Teruya K, Tachibana H, Shirahata S, Murakami H
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Biotherapy. 1993;7(2):115-23. doi: 10.1007/BF01877735.
A fermented milk, Kefir, contains an active substance which enhances IFN-beta secretion of a human osteosarcoma line MG-63 treated with a chemical inducer, poly I: poly C. The active substance in the fermented milk was identified to be sphingomyelin (SpM) by a combined use of a fast atom bombardment mass spectrometry (FAB-MS) and a fast atom bombardment tandem mass spectrometry (FAB-MS/MS). SpM from fermented milk (F-SpM) was a mixture of four molecular species of SpMs having C21-, C22-, C23- and C24-fatty acids. F-SpM enhanced the IFN secretion 14 times, SpMs from other sources also enhanced moderately (2-3 times). Sphingosine and lysosphingomyelin also enhanced the activity but ceramide and cerebroside did not.
一种发酵乳——开菲尔，含有一种活性物质，该物质可增强经化学诱导剂聚肌苷酸:聚胞苷酸（poly I: poly C）处理的人骨肉瘤细胞系MG - 63的β-干扰素分泌。通过联合使用快原子轰击质谱（FAB - MS）和快原子轰击串联质谱（FAB - MS/MS），确定发酵乳中的活性物质为鞘磷脂（SpM）。发酵乳中的鞘磷脂（F - SpM）是具有C21 -、C22 -、C23 -和C24 -脂肪酸的四种鞘磷脂分子种类的混合物。F - SpM使干扰素分泌增强了14倍，其他来源的鞘磷脂也有适度增强（2 - 3倍）。鞘氨醇和溶血鞘磷脂也增强了该活性，但神经酰胺和脑苷脂则没有。
