Istituto di Ricerche Farmacologiche "Mario Negri", Via Eritrea 62, 20157, Milan, Italy.
Cytotechnology. 1995 Oct;17(3):185-91. doi: 10.1007/BF00749656.
Clonogenic assay is one of the most sensitive assays, widely used to evaluate the effects of antineoplastic agentsin vitro. A computer program was developed on an IBAS 2.0 Image Analysis System for automated quantiation of cell colonies and clone area on Petri dishes. The sensitivity of the clonogenic assay can be greatly increased by evaluating the mean area of the clones. The program gives an objective, accurate and fast evaluation of large samples. It is simple to use and offers a high degree of flexibility. Special algorithms and techniques have been implemented for good quantitation of both connected and well-separated colonies and to reduce the background noise and the general error rate. The principles and solutions presented are applicable to any other image analysis system.
集落形成分析是最敏感的检测方法之一，广泛用于评估抗肿瘤药物的体外作用。我们在 IBAS 2.0 图像分析系统上开发了一个计算机程序，用于对培养皿上的细胞集落和克隆面积进行自动定量分析。通过评估克隆的平均面积，可以大大提高集落形成分析的灵敏度。该程序可以对大量样本进行客观、准确和快速的评估。它使用简单，并且具有高度的灵活性。我们实施了特殊的算法和技术，以便对连接的和良好分离的集落进行良好的定量，并降低背景噪声和总体错误率。所提出的原理和解决方案适用于任何其他图像分析系统。
