Laboratoire de Pharmacologie Cellulaire, Ecole Pratique des Hautes Etudes, 15 Rue de l'Ecole de Médecine, 75006, Paris, France.
Cytotechnology. 1988 Feb;1(2):123-32. doi: 10.1007/BF00146812.
Primary cultures of rabbit articular chondrocytes have been subcultured within three-dimensional (3D) collagen gels. Under these conditions, the cells remained viable and divided, but with a lower proliferation rate than that observed in control monolayer cultures. Flow cytometric analysis of progression of the cells into the cell cycle has confirmed and extended these findings. Also the cellular volume was decreased in 3D-culture, being in the same range as thein vivo size of cartilage cells. Specific staining for proteoglycans and type II collagen immunolocalization on sections of gels showed the expression of differentiated phenotypes and revealed the accumulation of these matrix components in the immediate surroundings of the cells. The use of Ultroser G (a serum substitute) improved the conditions for 3D- culture of rabbit articular chondrocytes.
已将兔关节软骨细胞进行原代培养，并在三维（3D）胶原凝胶中进行传代培养。在这些条件下，细胞仍然存活并分裂，但增殖速度低于对照单层培养。细胞周期进程的流式细胞分析证实并扩展了这些发现。此外，3D 培养中的细胞体积减小，与体内软骨细胞的大小相同。凝胶切片中对蛋白聚糖和 II 型胶原的特异性染色显示出分化表型的表达，并揭示了这些基质成分在细胞周围的积累。使用 Ultroser G（一种血清替代品）改善了兔关节软骨细胞 3D 培养的条件。
