Adolphe M, Froger B, Ronot X, Corvol M T, Forest N
Exp Cell Res. 1984 Dec;155(2):527-36. doi: 10.1016/0014-4827(84)90212-x.
The complexity and the variations in the efficiency of different batches of serum stimulated the preparation of a serum-free medium which could promote not only growth, but also the differentiation properties of rabbit articular chondrocytes in culture. The serum-free medium (SFM) developed in this study contained insulin, transferrin, Na-selenite, human fibronectin bovine serum albumin (BSA), brain growth factor (BGF) or fibroblast growth factor (FGF), hydrocortisone and multiplication stimulating activity (MSA). Primary or secondary cultures of chondrocytes in such a medium attained a proliferation rate equal to 70-80% of that obtained with chondrocytes grown in a serum control medium. The deletion of various factors from SFM indicates that BGF or FGF are the most stimulating of growth factors. Insulin was beneficial when used individually; when combined with BGF or FGF, they had a synergistic effect on cell proliferation. MSA seemed not to play any role in chondrocyte growth in culture. The SFM medium did not modify either the morphology or the progression of cells into the cell cycle. It moreover allowed the maintenance of the specific function of chondrocytes to synthesize type II collagen.
不同批次血清的复杂性及效率差异促使人们制备一种无血清培养基，该培养基不仅能促进培养的兔关节软骨细胞生长，还能促进其分化特性。本研究开发的无血清培养基（SFM）含有胰岛素、转铁蛋白、亚硒酸钠、人纤连蛋白、牛血清白蛋白（BSA）、脑生长因子（BGF）或成纤维细胞生长因子（FGF）、氢化可的松及增殖刺激活性（MSA）。在此培养基中进行软骨细胞的原代或传代培养，其增殖速率达到在血清对照培养基中生长的软骨细胞增殖速率的70 - 80%。从SFM中去除各种因子表明，BGF或FGF是最具刺激作用的生长因子。单独使用胰岛素有益；与BGF或FGF联合使用时，它们对细胞增殖具有协同作用。MSA似乎在培养的软骨细胞生长中不起任何作用。SFM培养基既未改变细胞形态，也未改变细胞进入细胞周期的进程。此外，它能维持软骨细胞合成Ⅱ型胶原的特定功能。
