Biotechnology Research Laboratory, Teijin Limited, 191, Hino, Japan.
Cytotechnology. 1988 Feb;1(2):171-8. doi: 10.1007/BF00146818.
We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media.The concentration of IgG after 48 days culture of H2 cells in ITES-eRDF reached 2 mg/ml and the purity of IgG in culture fluid was 61%. In addition, high molecular weight components in serum-free media, such as transferrin or BSA, could be reduced to 5% of the original concentration.
我们开发了一种可回收高分子量组分的高密度和高产物浓度培养系统。该培养系统采用无血清限定培养基，对鼠-人杂交瘤 H2 和 V6 细胞进行培养，实现了单克隆抗体的高浓度生产。在 ITES-eRDF 中培养 H2 细胞 48 天后，IgG 的浓度达到 2mg/ml，培养液中 IgG 的纯度为 61%。此外，无血清培养基中的高分子量组分，如转铁蛋白或 BSA，可降低至原始浓度的 5%。
