通过高密度灌注培养生产人鼠嵌合抗体。

Production of human-mouse chimeric antibody by high cell density perfusion culture.

机构信息

Biotechnology Research Laboratory, Teijin Limited, 191, Hino, Tokyo, Japan.

出版信息

Cytotechnology. 1989 May;2(2):95-101. doi: 10.1007/BF00386141.

Abstract

Two mouse myeloma cell lines which were transfected with chimeric mouse variable-human constant immunoglobulin heavy and light chain genes have been cultured at high cell density in a settling perfusion culture vessel to produce chimeric antibody specific for human common acute lymphocytic leukemia antigen (cALLA).J558L transfectant proliferated well in a serum-free medium (ITES-eRDF) to a viable cell density of 3.7×10(7) cells/ml and produced chimeric antibody to a maximum value of 60 μg/ml in 120 ml scale vessel. X63Ag8.653 transfectant reached a density of 1.9×10(7) cells/ml in 1.2 I scale vessel in serum supplemented medium (10% FCS-eRDF) and produced chimeric antibody which consisted of chimeric gamma and chimeric kappa chains to a maximum value of 5.8 μg/ml.

摘要

已将转染嵌合鼠可变区-人恒定区免疫球蛋白重链和轻链基因的两种鼠骨髓瘤细胞系在沉降式灌注培养容器中于高细胞密度下培养，以生产针对人普通急性淋巴细胞白血病抗原（cALLA）的嵌合抗体。J558L 转染子在无血清培养基（ITES-eRDF）中生长良好，在 120 毫升规模容器中达到 3.7×10(7)个细胞/ml 的活细胞密度，并产生最大 60 μg/ml 的嵌合抗体。X63Ag8.653 转染子在补充有血清的培养基（10% FCS-eRDF）中 1.2 I 规模容器中达到 1.9×10(7)个细胞/ml 的密度，并产生由嵌合 γ 和嵌合 κ 链组成的嵌合抗体，最大达到 5.8 μg/ml。