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初始培养条件影响 HepG2 细胞对过度机械搅拌的敏感性。

Initial culture conditions affect the sensitivity of HepG2 cells to excessive mechanical agitation.

机构信息

Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul, Korea.

出版信息

Cytotechnology. 1989 May;2(2):135-40. doi: 10.1007/BF00386146.

DOI:10.1007/BF00386146
PMID:22358626
Abstract

Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.

摘要

肝癌细胞 HepG2 在微载体上即使在相对较高的搅拌速度下也能正常生长,如果有足够的时间用于细胞附着和黏附的话。然而,如果在初始细胞附着后不久就施加高搅拌速度,则会延迟生长速度。这种对机械搅拌的敏感性似乎取决于接种细胞密度。

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1
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Cytotechnology. 1989 May;2(2):135-40. doi: 10.1007/BF00386146.
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本文引用的文献

1
Selection of microcarrier diameter for the cultivation of mammalian cells on microcarriers.用于在微载体上培养哺乳动物细胞的微载体直径的选择。
Biotechnol Bioeng. 1987 Sep;30(4):548-57. doi: 10.1002/bit.260300412.
2
Attachment and growth of mammalian cells on microcarriers with different ion exchange capacities.哺乳动物细胞在具有不同离子交换能力的微载体上的附着与生长
Biotechnol Bioeng. 1987 Jun;29(9):1155-63. doi: 10.1002/bit.260290917.
3
Hydrodynamic effects on animal cells grown in microcarrier cultures.微载体培养中流体动力学对动物细胞的影响。
Biotechnol Bioeng. 1987 Jan;29(1):130-41. doi: 10.1002/bit.260290117.
4
Production of cell-derived products: virus and interferon.细胞衍生产品的生产:病毒与干扰素。
Ann N Y Acad Sci. 1981;369:47-59. doi: 10.1111/j.1749-6632.1981.tb14176.x.