Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Seoul, Korea.
Cytotechnology. 1989 May;2(2):135-40. doi: 10.1007/BF00386146.
Hepatoma cells, HepG2, grew normally on microcarriers even at a relatively high agitation rate if sufficient time was allowed for cell attachment and adhesion. However, if a high agitation rate was applied shortly after initial cell attachment, the growth rate was retarded. This sensitivity to mechanical agitation appears to be dependent on the inoculation cell density.
肝癌细胞 HepG2 在微载体上即使在相对较高的搅拌速度下也能正常生长,如果有足够的时间用于细胞附着和黏附的话。然而,如果在初始细胞附着后不久就施加高搅拌速度,则会延迟生长速度。这种对机械搅拌的敏感性似乎取决于接种细胞密度。