Department of Vaccine Research and Development, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Via A. Bianchi 7, 25100, Brescia, Italy.
Cytotechnology. 1993 Jan;11(Suppl 1):S106-8. doi: 10.1007/BF00746070.
The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell concentrations at the end of culturing, the progressive reduction of spreading on surfaces and an abnormal expression of the α5β1 integrin were found to be correlated with the number of passages in suspension culture. The observed modifications in the normal course of the cell-cycle and in the expression of some surface proteins point at a possible mechanism of virus damages arising from defective cellular functions.
BHK-21 悬浮细胞培养具有经济便捷的优点，但可能产生免疫原性较弱的口蹄疫病毒。通过研究细胞培养的少数基因型和表型特征，我们获得了一些有用的认识。多倍体群体的出现、培养结束时更高的细胞浓度、在表面上扩散能力的逐渐降低以及α5β1 整合素的异常表达与悬浮培养中的传代数有关。观察到细胞周期的正常过程和一些表面蛋白表达的改变，提示可能存在由于细胞功能缺陷导致病毒损伤的机制。
