Grigera P R, Tisminetzky S G, Lebendiker M B, Periolo O H, La Torre J L
Centro de Virologia Animal (CEVAN-CONICET), Serrano, Argentina.
Virology. 1988 Aug;165(2):584-8. doi: 10.1016/0042-6822(88)90602-2.
A 43kDa cellular polypeptide (P43), which comigrates with host-cell actin in both SDS-PAGE and isoelectrofocusing slab gels, was found associated to 140 S aphthoviral particles purified from BHK 21 cells labeled with [35S]methionine prior to infection. Ultracentrifugation analysis of disrupted virions demonstrates that polypeptide P43 is not associated to VP1-3 containing 12 S subunits but remains, like viral polypeptide VP4, at the top of the sucrose gradients. In addition, in vitro iodination or trypsin treatment show that P43 is protected from the action of both procedures and therefore supports the hypothesis that host-cell polypeptide P43 is located within the viral particles.
一种43kDa的细胞多肽（P43），在SDS-PAGE和等电聚焦平板凝胶中均与宿主细胞肌动蛋白迁移率相同，它与从感染前用[35S]甲硫氨酸标记的BHK 21细胞中纯化的140S口蹄疫病毒颗粒相关。对破碎病毒粒子的超速离心分析表明，多肽P43不与含有12S亚基的VP1-3相关，而是像病毒多肽VP4一样，保留在蔗糖梯度的顶部。此外，体外碘化或胰蛋白酶处理表明，P43不受这两种处理的影响，因此支持宿主细胞多肽P43位于病毒粒子内的假说。
