Department of Genetics and Microbiology, Univ. of Pavia, 27100, Pavia, Italy.
Cytotechnology. 1993 Jan;11(Suppl 1):S123-5. doi: 10.1007/BF00746075.
PCR with random primers (RAPD analysis) performed on the DNA of embryogenic and non-embryogenic suspension cultured rice and of transformed rice plants allows the evaluation of the extent of DNA changes in the different biological materials. This is thus suggested as a convenient approach, in combination with restriction analysis and Southerns blotting, to evaluate the integrity of the foreign gene, the stability of the insertion site and the stability of the whole genome.
使用随机引物聚合酶链反应（RAPD 分析）对胚性和非胚性悬浮培养水稻以及转化水稻植株的 DNA 进行分析，可以评估不同生物材料中 DNA 变化的程度。因此，这被建议作为一种方便的方法，与限制分析和 Southern 印迹相结合，用于评估外源基因的完整性、插入位点的稳定性和整个基因组的稳定性。
