Bao P H, Castiglione S, Giordani C, Li W, Wang G, Datta S K, Datta K, Potrykus I, Sala F
Department of Genetics and Microbiology, Univ. of Pavia, Italy.
Cytotechnology. 1993;11 Suppl 1:S123-5.
PCR with random primers (RAPD analysis) performed on the DNA of embryogenic and non-embryogenic suspension cultured rice and of transformed rice plants allows the evaluation of the extent of DNA changes in the different biological materials. This is thus suggested as a convenient approach, in combination with restriction analysis and Southerns blotting, to evaluate the integrity of the foreign gene, the stability of the insertion site and the stability of the whole genome.
对胚性和非胚性悬浮培养水稻以及转基因水稻植株的DNA进行随机引物PCR（RAPD分析），可以评估不同生物材料中DNA变化的程度。因此，建议将其作为一种便捷的方法，与限制性分析和Southern杂交相结合，以评估外源基因的完整性、插入位点的稳定性以及整个基因组的稳定性。
