Dept. of Chemical Engineering, Via Diotisalvi 2, 56126, Pisa, Italy.
Cytotechnology. 1993 Jan;11(Suppl 1):S137-9. doi: 10.1007/BF00746079.
In vitro cytotoxicity (Neutral Red uptake, Kenacid Blue and MTT) and cytocompatibility (cell adhesion and proliferation) tests were applied to the biocompatibility study of a series of poly(ester-ether-ester) block copolymers of potential interest as biomaterials. Our results indicate that the copolymer extracts after 72 hours incubation with a 3T3 mouse fibroblast cell line do not induce significant toxic effects. Furthermore, human umbilical vein endothelial cells seeded on thin copolymer films show a normal pattern of growth. We conclude that thein vitro tests used are a valid instrument to evaluate the potential toxic action of synthetic materials on different cell compartments and that the tested materials seem to be promising for future applications in the field of biomedical devices.
体外细胞毒性（中性红摄取、肯酸蓝和 MTT）和细胞相容性（细胞黏附和增殖）测试应用于一系列具有生物材料应用潜力的聚酯醚酯嵌段共聚物的生物相容性研究。我们的结果表明，与 3T3 小鼠成纤维细胞系孵育 72 小时后，共聚物浸提物不会引起明显的毒性作用。此外，在薄共聚物膜上接种的人脐静脉内皮细胞显示出正常的生长模式。我们得出结论，体外测试是评估合成材料对不同细胞区室潜在毒性作用的有效工具，并且所测试的材料似乎有希望在生物医学设备领域得到进一步应用。
