Cascone M G, Tricoli M, Cerrai P, Sbarbati Del Guerra R
C.N.R. Centro Studi Processi Ionici, Pisa, Italy.
Cytotechnology. 1993;11 Suppl 1:S137-9.
In vitro cytotoxicity (Neutral Red uptake, Kenacid Blue and MTT) and cytocompatibility (cell adhesion and proliferation) tests were applied to the biocompatibility study of a series of poly(ester-ether-ester) block copolymers of potential interest as biomaterials. Our results indicate that the copolymer extracts after 72 hours incubation with a 3T3 mouse fibroblast cell line do not induce significant toxic effects. Furthermore, human umbilical vein endothelial cells seeded on thin copolymer films show a normal pattern of growth. We conclude that the in vitro tests used are a valid instrument to evaluate the potential toxic action of synthetic materials on different cell compartments and that the tested materials seem to be promising for future applications in the field of biomedical devices.
体外细胞毒性（中性红摄取法、肯那酸蓝法和MTT法）及细胞相容性（细胞黏附和增殖）测试应用于一系列具有潜在生物材料应用价值的聚（酯-醚-酯）嵌段共聚物的生物相容性研究。我们的结果表明，与3T3小鼠成纤维细胞系孵育72小时后的共聚物提取物未诱导明显的毒性作用。此外，接种在共聚物薄膜上的人脐静脉内皮细胞呈现出正常的生长模式。我们得出结论，所使用的体外测试是评估合成材料对不同细胞区室潜在毒性作用的有效手段，并且所测试的材料在生物医学设备领域的未来应用中似乎很有前景。
