Department of Biology and Technology, H.S. Raffaele Institute, 20132, Milano, Italy.
Cytotechnology. 1993 Jan;11(Suppl 1):S41-3. doi: 10.1007/BF00746050.
Cultured embryionic day 6 (E6) retinal neurons respond to laminin by making use of integrin receptors. We have recently shown that the laminin binding integrin receptor α6β1 is expressed in the chick retina on both retinal ganglion cells and other retinal neurons. Antibodies raised against a fusion protein containing a large fragment of the extracellular portion of the chick α6 integrin subunit dramatically inhibit the interactions between E6 retinal neurons and laminin. These data show that α6β1 functions as a laminin receptor in these cells. In previous work we have also shown that the levels of the mRNA for α6 decreases dramatically in retinal ganglion cells between E6 and E12. Data presented in this paper show that the decrease in α6 mRNA is not prevented by ablation of the optic tectum, indicating that contact with the target is not a major cause for this decrease.
培养的胚胎第 6 天（E6）视网膜神经元通过整合素受体对层粘连蛋白作出反应。我们最近发现，层粘连蛋白结合的整合素受体α6β1 在小鸡视网膜上的神经节细胞和其他视网膜神经元上均有表达。针对含有小鸡α6 整合素亚基胞外部分大片段的融合蛋白产生的抗体，强烈抑制 E6 视网膜神经元与层粘连蛋白之间的相互作用。这些数据表明，α6β1 在这些细胞中作为层粘连蛋白受体发挥作用。在以前的工作中，我们还发现α6 的 mRNA 水平在 E6 和 E12 之间的神经节细胞中急剧下降。本文提供的数据表明，视神经顶盖的缺失并不能阻止α6 mRNA 的减少，这表明与靶细胞的接触并不是导致这种减少的主要原因。
