悬浮哺乳动物细胞培养中的搅拌和气升效应。

Agitation and aeration effects in suspension mammalian cell cultures.

机构信息

Dept. of Infectious Diseases, The University of Sydney, 2006, NSW, Australia.

出版信息

Cytotechnology. 1989 Aug;2(3):163-70. doi: 10.1007/BF00133241.

Abstract

Three different hybridoma cell lines, grown in serum-free media with different levels of Pluronic F-68, were subjected to a shear force of 0.6 N m(-2). Some protective effect due to the polymer was found, indicating it to be a potentially useful adjuvant in serum-free media. Other observations of liquid and gas effects at the reactor level have been included here. A discussion of the difference between suspension and microcarrier cultures, in relation to hydrodynamic effects, is included.

摘要

三种不同的杂交瘤细胞系在含有不同浓度普朗尼克 F-68 的无血清培养基中生长，然后受到 0.6 N m(-2) 的剪切力。由于聚合物的存在，发现了一些保护作用，表明它可能是无血清培养基中的一种有用的佐剂。这里还包括了在反应器水平上对液体和气体影响的其他观察结果。讨论了悬浮培养和微载体培养之间的差异，以及与流体力学效应的关系。